Mouse MARCO APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2956A
Mouse MARCO APC-conjugated Antibody in Data
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Mouse MARCO APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MARCO in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 579511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MARCO
Gln70-Ser518
Accession # Q60754
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of MARCO antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of MARCO in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse MARCO APC-conjugated Monoclonal Antibody (Catalog # FAB2956A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of MARCO antibody in J774 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry. J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO APC-conjugated Monoclonal Antibody (Catalog # FAB2956A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MARCO

Mouse MARCO is a type II transmembrane glycoprotein belonging to the class A scavenger receptor family. It is constitutively expressed in subsets of macrophages found in the marginal zone of the spleen, the peritoneum, and the medullary cord of lymph nodes. The extracellular domains of MARCO form a 220 kDa disulfide-linked homotrimer on the cell surface. MARCO binds both gram positive and gram negative bacteria, as well as oxidized low-density lipoprotein. The amino acid sequence of mouse MARCO extracellular domain is 69% identical to that of human MARCO.

Long Name
Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
Entrez Gene IDs
8685 (Human); 17167 (Mouse)
Alternate Names
macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2

Product Datasheets

Citations for Mouse MARCO APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Small molecule-based inhibition of MEK1/2 proteins dampens inflammatory responses to malaria, reduces parasite load, and mitigates pathogenic outcomes
    Authors: X Wu, KK Dayanand, RP Thylur, CC Norbury, DC Gowda
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Phagosomal Acidification Prevents Macrophage Inflammatory Cytokine Production to Malaria, and Dendritic Cells Are the Major Source at the Early Stages of Infection: IMPLICATION FOR MALARIA PROTECTIVE IMMUNITY DEVELOPMENT.
    Authors: Wu X, Gowda N, Gowda D
    J Biol Chem, 2015;290(38):23135-47.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Mouse MARCO APC-conjugated Antibody

Average Rating: 4 (Based on 1 Review)

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Mouse MARCO APC-conjugated Antibody
By Anonymous on 12/07/2018
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human

Binds really nicely to muMarco transiently expressing cells, does not bind to untransfected cells.