Mouse MARCO Fluorescein-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse MARCO in direct ELISAs.
  • Source
    Monoclonal Rat IgG1 Clone # 579511
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MARCO
    Accession # Q60754
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MARCO in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse MARCO Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2956F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MARCO

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein that belongs to the class A scavenger receptor family (1). Mouse MARCO consists of a 48 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 449 aa extracellular domain (ECD) that includes a stalk region, a collagen-like region, and one SRCR domain (2). Within the ECD, mouse MARCO shares 69% and 86% aa sequence identity with human and rat MARCO, respectively. It shares 18%‑28% aa sequence identity with other mouse class A scavenger receptors CL-P1, SCARA3, SCARA5, and SR-A1/MSR. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (2, 3). Its expression is induced on Kupffer cells and alveolar macrophages by microbial infection, chemical irritants, and Th1 polarizing factors (3‑5). MARCO binds LPS, lipoteichoic acid, and other determinants on Gram positive and Gram negative bacteria (2, 6‑8). It also binds modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO2 (2, 9‑11). MARCO is required for the organization of the splenic marginal zone and the interaction of local macrophages and B cells (12, 13). The SRCR domain mediates binding of MARCO to its various ligands (3, 12), while the collagen-like region mediates assembly into a disulfide-linked trimeric molecule (2, 7). MARCO ligation induces, but is not required for the production of IL-12, NO, or TNF-alpha by macrophages (5, 6, 9). MARCO knockout mice show a reduced clearance of bacterial infections, reduced mast cell mediated silicosis, increased pulmonary inflammation, and increased sensitivity to ozone induced lung damage (4, 9, 14‑16).

  • References:
    1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
    2. Elomaa, O. et al. (1995) Cell 80:603.
    3. Van der Laan, L.J.W. et al. (1999) J. Immunol. 162:939.
    4. Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
    5. Jozefowski, S. et al. (2005) J. Immunol. 175:8032. 
    6. Mukhopadhyay, S. et al. (2006) Eur. J. Immunol. 36:940. 
    7. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. 
    8. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767. 
    9. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
    10. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
    11. Hamilton, Jr. R.F. et al. (2006) J. Biol. Chem. 281:34218.
    12. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.
    13. Chen, Y. et al. (2005) J. Immunol. 175:8173.
    14. Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
    15. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
    16. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.
  • Long Name:
    Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
  • Entrez Gene IDs:
    8685 (Human); 17167 (Mouse)
  • Alternate Names:
    macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; member 2; SCARA2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Fluorescein‑conjugated Control Antibody

Ctrl IC005F 2
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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