Mouse MARCO Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2956F
Mouse MARCO Fluorescein-conjugated Antibody in Data
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Mouse MARCO Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MARCO in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 579511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MARCO
Gln70-Ser518
Accession # Q60754
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of MARCO antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of MARCO in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse MARCO Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2956F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of MARCO antibody in Mouse Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of MARCO in Mouse Dendritic Cells by Flow Cytometry. Mouse splenocytes were differentiated to dendritic cells by culturing with recombinant mouse IL-4 (Catalog # 404-ML, 20 ng/ml) and recombinant mouse GM-CSF (Catalog # 415-ML, 40 ng/ml) for 6 days, then stained with (A) Rat Anti-Mouse MARCO Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2956F) or (B) isotype control antibody (Catalog # IC005F) and Rat anti-Mouse CD11c Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB69501R). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of MARCO antibody in J774 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry. J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO CFS-conjugated Monoclonal Antibody (Catalog # FAB2956F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MARCO

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein that belongs to the class A scavenger receptor family (1). Mouse MARCO consists of a 48 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 449 aa extracellular domain (ECD) that includes a stalk region, a collagen-like region, and one SRCR domain (2). Within the ECD, mouse MARCO shares 69% and 86% aa sequence identity with human and rat MARCO, respectively. It shares 18%‑28% aa sequence identity with other mouse class A scavenger receptors CL-P1, SCARA3, SCARA5, and SR-A1/MSR. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (2, 3). Its expression is induced on Kupffer cells and alveolar macrophages by microbial infection, chemical irritants, and Th1 polarizing factors (3‑5). MARCO binds LPS, lipoteichoic acid, and other determinants on Gram positive and Gram negative bacteria (2, 6‑8). It also binds modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO2 (2, 9‑11). MARCO is required for the organization of the splenic marginal zone and the interaction of local macrophages and B cells (12, 13). The SRCR domain mediates binding of MARCO to its various ligands (3, 12), while the collagen-like region mediates assembly into a disulfide-linked trimeric molecule (2, 7). MARCO ligation induces, but is not required for the production of IL-12, NO, or TNF-alpha by macrophages (5, 6, 9). MARCO knockout mice show a reduced clearance of bacterial infections, reduced mast cell mediated silicosis, increased pulmonary inflammation, and increased sensitivity to ozone induced lung damage (4, 9, 14‑16).

References
  1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
  2. Elomaa, O. et al. (1995) Cell 80:603.
  3. Van der Laan, L.J.W. et al. (1999) J. Immunol. 162:939.
  4. Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
  5. Jozefowski, S. et al. (2005) J. Immunol. 175:8032. 
  6. Mukhopadhyay, S. et al. (2006) Eur. J. Immunol. 36:940. 
  7. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. 
  8. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767. 
  9. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
  10. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
  11. Hamilton, Jr. R.F. et al. (2006) J. Biol. Chem. 281:34218.
  12. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.
  13. Chen, Y. et al. (2005) J. Immunol. 175:8173.
  14. Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
  15. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
  16. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.
Long Name
Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
Entrez Gene IDs
8685 (Human); 17167 (Mouse)
Alternate Names
macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2

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Mouse MARCO Fluorescein-conjugated Antibody
By Anonymous on 01/12/2018
Application: Flow Sample Tested: IC-21 mouse macrophage cell line Species: mouse macrophage,Mouse