Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Transgenic Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 579511
View all MARCO Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MARCO
Gln70-Ser518
Accession # Q60754

Specificity

Detects mouse MARCO in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse MARCO Antibody

Detection of MARCO antibody in J774 Mouse Cell Line antibody by Flow Cytometry

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry

J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO Monoclonal Antibody (Catalog # MAB2956) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MARCO Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: J774 Mouse cell line

Reviewed Applications

Read 1 review rated 4 using MAB2956 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MARCO

Mouse MARCO is a type II transmembrane glycoprotein belonging to the class A scavenger receptor family. It is constitutively expressed in subsets of macrophages found in the marginal zone of the spleen, the peritoneum, and the medullary cord of lymph nodes. The extracellular domains of MARCO form a 220 kDa disulfide-linked homotrimer on the cell surface. MARCO binds both gram positive and gram negative bacteria, as well as oxidized low-density lipoprotein. The amino acid sequence of mouse MARCO extracellular domain is 69% identical to that of human MARCO.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Alternate Names

SCARA2

Entrez Gene IDs

8685 (Human); 17167 (Mouse)

Gene Symbol

MARCO

UniProt

Q60754

Additional MARCO Products

Product Documents for Mouse MARCO Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse MARCO Antibody

For research use only

Customer Reviews for Mouse MARCO Antibody (1)

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  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: A-673 human Ewing's sarcoma cell line
    Species: Mouse
    Verified Customer | Posted 03/10/2021

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FAQs

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail