Mouse MARCO Antibody

Catalog # Availability Size / Price Qty
MAB2956-100
MAB2956-SP

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Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry
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Mouse MARCO Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MARCO in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 579511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MARCO
Gln70-Ser518
Accession # Q60754
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MARCO antibody in J774 Mouse Cell Line antibody by Flow Cytometry View Larger

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO Monoclonal Antibody (Catalog # MAB2956) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MARCO

Mouse MARCO is a type II transmembrane glycoprotein belonging to the class A scavenger receptor family. It is constitutively expressed in subsets of macrophages found in the marginal zone of the spleen, the peritoneum, and the medullary cord of lymph nodes. The extracellular domains of MARCO form a 220 kDa disulfide-linked homotrimer on the cell surface. MARCO binds both gram positive and gram negative bacteria, as well as oxidized low-density lipoprotein. The amino acid sequence of mouse MARCO extracellular domain is 69% identical to that of human MARCO.

Long Name
Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
Entrez Gene IDs
8685 (Human); 17167 (Mouse)
Alternate Names
macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2

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Mouse MARCO Antibody
By Anonymous on 03/10/2021
Application: IP Sample Tested: A-673 human Ewing's sarcoma cell line Species: Mouse