Mouse Mast Cell Protease-6/Mcpt6 Antibody

Catalog # Availability Size / Price Qty
MAB4288
MAB4288-SP
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Mouse Mast Cell Protease-6/Mcpt6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Mast Cell Protease‑6/Mcpt6 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 286828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑6/Mcpt6
Ile32-Ser276
Accession # P21845
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (Catalog # 3736-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (1 µg/mL, Catalog # 3736-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-6/Mcpt6

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast Cell Protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, MCP-6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, MCP-6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). MCP-6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

References
  1. Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535.
  2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
  3. Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.
Entrez Gene IDs
7177 (Human); 17229 (Mouse); 29268 (Rat)
Alternate Names
Mast Cell Protease6; Mast Cell Protease-6; Mcpt6

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