Mouse MBL-2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2208
Product Details
Citations (2)
FAQs
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Mouse MBL-2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MBL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse MBL-1 and recombinant human MBL is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MBL-2
Glu19-Asp244
Accession # P41317
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse MBL‑2 (Catalog # 2208-MB)

Mouse MBL-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse MBL‑2 Antibody (Catalog # AF2208)

Standard: Recombinant Mouse MBL-2 Protein (Catalog # 2208-MB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBL-2

Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which binds to an array of carbohydrate patterns on pathogen surfaces (1, 2). Collectin family members share common structural features: a cysteine rich amino-terminal domain, a collagen-like region, an alpha -helical coiled-coil neck domain and a carboxy terminal C-type (Ca++-dependent) lectin or carbohydrate recognition domain (CRD). MBL homotrimerizes to form a structural unit joined byN-terminal disulfide bridges. These homotrimers further associates into oligomeric structures of up to 6 units. Whereas two forms of MBL proteins (MBL-1, also known as S-MBP or MBL-A and MBL-2, also known as L-MBP or MBL-C) exist in rodents and other animals, only one functional MBL protein is present in humans. Mouse MBL-2 shares about 52% and 60% amino acid sequence identity with mouse MBL-1 and human MBL, respectively.

In mouse, MBL-1 and MBL-2 are the only collectins that can activate complement via the lectin complement pathway (1, 2). Serum oligomeric MBL associates with MBL-associated serine protease (MASP) proenzymes. The MBL-MASP proenzyme complex preferentially interact with sugar patterns containing mannose, glucose, L‑fucose, or N-acetyl-glucosamine present at a terminal nonreducing postion on the cell surface of various pathogens and certain tumor cells. This interaction induces pro-enzyme activation and the triggering of the complement cascade, resulting in opsonization and pathogen removal via humoral and cellular immune responses. MBL does not recognize self-components or glycoproteins from other higher animals due to the presence of terminal sialic acid or galactose that interrupts the repeating carbohydrate structures (3). A number of membrane receptors for MBL, including C1q phagocytic receptor (C1qRp), calreticulin (also known as C1qR), and CR1 (CD35), have been described. Interactions with these receptors may also be important in stimulating phagocytosis (1, 2).

Mouse MBL-1 and MBL-2 are produced primarily in the liver and are secreted into the blood stream. In addition, mouse MBL-1 is also expressed in lung, kidney, and testis while MBL-2 is expressed in kidney, thymus, and small intestine (1, 4, 5).

References
  1. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
  2. Fujita, R. et al. (2004) Immunol. Rev. 198:185.
  3. Saevarsdottir, S. et al. (2004) Scand. J. Immnunol. 60:23.
  4. Uemura, K. et al. (2002) J. Immunol. 169:6945.
  5. Wagner, S. et al. (2003) J. Immunol. 170:1462.
Long Name
Mannose Binding Lectin 2
Entrez Gene IDs
17195 (Mouse)
Alternate Names
L-MBP; mannose-binding protein C; L-MBP; mannose-binding lectin (protein C) 2; MBL; MBL-2; MBL-C; MBP-C

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Citations for Mouse MBL-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation.
    Authors: Foo, Suan-Sin, Chen, Weiqiang, Taylor, Adam, Sheng, Kuo-Chin, Yu, Xing, Teng, Terk-Shi, Reading, Patrick, Blanchard, Helen, Garlanda, Cecilia, Mantovani, Alberto, Ng, Lisa F P, Herrero, Lara J, Mahalingam, Suresh
    PLoS Pathog, 2015-02-19;11(2):e1004649.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  2. Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis.
    Authors: Green RS, Stone EL, Tenno M, Lehtonen E, Farquhar MG, Marth JD
    Immunity, 2007-08-02;27(2):308-20.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot

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