Mouse MD-1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF130

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Mouse MD-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MD-1 in Western blots. In Western blots, approximately 100% cross-reactivity with human MD-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MD-1
Asp20-Ser162
Accession # O88188
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse MD‑1 (Catalog # 130-MD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MD-1

MD-1 is a secreted glycoprotein that was originally identified as a v-myb-regulated gene from avian myeloleukemia virus-transformed chicken myeloblasts (1). The mouse homologue of chicken MD‑1 (also known as lymphocyte antigen 86) was subsequently discovered as a molecule that is associated with RP105, a type I transmembrane glycoprotein with extracellular leucine-rich repeats (LRR) typically found in Toll-like receptor (TLR) family members. However, RP105 has a short cytoplasmic tail and lacks the Toll-IL-1 R (TIR) domain that defines the IL-1 R/TLR superfamily (2‑4). RP105 plays an important role in B-cell activation by bacterial lipopolysaccharide (LPS). It is expressed primarily on mature B cells, dendritic cells and macrophages (4).

Mouse MD-1 cDNA encodes a 162 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and two potential N‑linked glycosylation sites. It shares 40% and 66% aa sequence identity with chicken and human MD-1 respectively (2, 3). MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney. MD-1 is required for efficient RP105 cell surface expression and function (2‑5). Cell surface RP105/MD-1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B-cells. Activation of the RP105 complex has been shown to protect against apoptosis, induce B-cell proliferation and upregulate B7.2, a co-stimulatory molecule (5, 6). Since MD-1 is also expressed in liver and brain where RP105 is absent, it has been speculated that MD-1 can also be associated with other LRR-containing molecules, or have additional functions outside the immune system (6).

References
  1. Burk, O. and K. Klempnauer (1991) EMBO J. 10:3713.
  2. Miyake, K. et al. (1998) J. Immun. 161:1348.
  3. Miura, Y. et al. (1998) Blood 92:2815.
  4. Miyake, K. et al. (1995) J. Immunol. 154:3333.
  5. Nagai, Y. et al. (2002) Blood 99:1699.
  6. Ogata, H. et al. (2000) J. Exp. Med. 192:23.
Long Name
Myeloid Differentiation Protein 1/Lymphocyte Antigen 86
Entrez Gene IDs
9450 (Human); 17084 (Mouse)
Alternate Names
LY86; Ly-86; lymphocyte antigen 86; MD1; MD-1; MMD-1; Protein MD-1; RP105-associated

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