Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Bovine

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Mimecan Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mimecan
Ala20-Phe298
Accession # Q62000

Specificity

Detects mouse Mimecan in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Mimecan is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Mimecan Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Mimecan (Catalog # 2949-MC)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mimecan

Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine rich repeats in the central domain, which is flanked by N- and C-terminal cysteine‑rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C‑terminal amino acid residues) and Mimecan (containing 223 most C‑terminal residues). Mouse Mimecan shares approximately 94% and 85% amino acid sequence identity with rat and human Mimecan, respectively.

Alternate Names

KSPG25, OGN, OIF, SLRR3A

Entrez Gene IDs

4969 (Human); 18295 (Mouse)

Gene Symbol

OGN

UniProt

Q62000

Additional Mimecan Products

Product Documents for Mouse Mimecan Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Mimecan Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Mouse Mimecan Antibody

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