Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 444008
View all MS4A4B Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with mouse MS4A4B
Met1-Val226
Accession # NP_068364

Specificity

Detects mouse MS4A4B. Stains mouse MS4A4B transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse MS4A4B Antibody

Detection of MS4A4B antibody in Mouse Thymocytes antibody by Flow Cytometry.

Detection of MS4A4B in Mouse Thymocytes by Flow Cytometry.

Mouse thymocytes were stained with Rat Anti-Mouse MS4A4B Monoclonal Antibody (Catalog # MAB4504, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Applications for Mouse MS4A4B Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse thymocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MS4A4B

MS4A4B, also called Chandra, is a member of the MS4A family of four-transmembrane proteins that includes CD20. It is expressed in immature CD4-CD8- T cells, and later in mature Th1 but not Th2 cells. MS4A4B is enriched in lipid rafts of activated T cells, and overexpression enhances Th1 cytokine expression, indicating a possible role in cell signaling. Mouse MS4A4B shares 69% overall amino acid (aa) identity with rat, and low (< 40%) aa identity with human MS4A4B.

Long Name

Membrane-spanning 4-domains, Subfamily A, Member 4B

Alternate Names

Chandra, Ly116

Entrez Gene IDs

60361 (Mouse)

Gene Symbol

MS4A4B

UniProt

NP_068364

Additional MS4A4B Products

Product Documents for Mouse MS4A4B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MS4A4B Antibody

For research use only

Citations for Mouse MS4A4B Antibody

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