Mouse Nectin-2/CD112 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Nectin-2/CD112 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Nectin-2 or recombinant mouse CD155/PVR is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 829038
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Nectin-2/CD112
    Gln32-Gly351 (predicted)
    Accession # P32507
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Nectin-2/CD112 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Nectin‑2/CD112 Monoclonal Antibody (Catalog # MAB3869, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nectin-2/CD112

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that control cell adhesion, proliferation, and migration (1, 2, 3). The name Nectin derives from the Latin word necto, which means “to connect”. The Nectin family contains four members (Nectin-1 to -4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma  which is secreted), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been given the alternate name of poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Mouse Nectin-2 is a 70 to 78 kDa type I TM glycoprotein that is found on a variety of cell types (4, 5). It has two splice forms (4, 6, 7). Nectin-2 alpha /PRR2 is a 65 kDa short form and is synthesized as a 467 amino acid precursor. It contains a 31 aa signal sequence, a 315 aa extracellular domain (ECD), a 28 aa TM segment, and a 93 aa cytoplasmic region. The ECD contains one N-terminal V-type Ig domain and two 85‑95 aa C2-type Ig-like domains (aa 153‑337) (8). The V-domain is believed to mediate Nectin binding to its ligands (9). A long, 78 kDa, 530 aa isoform of mouse Nectin-2 (Nectin-2δ) also exists. It has the same signal sequence and extracellular domain as Nectin-2 alpha  (aa 1‑338), but differs in the TM segment (21 aa in length) and cytoplasmic region (159 aa in length) (4, 6, 7). Mouse Nectin-2 ECD (aa 32 - 338) shares 72%, 77% and 95% aa identity with the ECD in human, canine and rat Nectin-2, respectively. Nectin-2 is known to bind pseudorabies virus, and herpes simplex virus-2 (HSV-2). It also binds select HSV-1 strains. It does not bind poliovirus (1, 10, 11). As a cell adhesion molecule, Nectin-2 will form cis‑homodimers (same cell) and trans-homodimers (across cells). Nectin-2 will not cis‑dimerize with other Nectins, but will trans-heterodimerize with Nectin-3 and CD266/DNAM-1 (1, 3, 11, 12, 13). Nectin-2 is found concentrated at cell-to-cell interfaces, and is presumed to contribute to tight and adherens junction formation (14). Through its interaction with NK and T cell expressed DNAM-1, it also promotes lymphocyte cytotoxicity and cytokine secretion against both tumors and dendritic cells (DC) expressing Nectin-2 (15, 16). In the case of DC, this may be a mechanism whereby the immune system eliminates DC that are inefficient at antigen presentation. Nectin-2 is expressed on epithelium, endothelial cells, Sertoli cells, monocytes, dendritic cells, granulosa cells, mast cells, eosinophils and fibroblasts.

  • References:
    1. Takai, Y. and H. Nakanishi (2003) J. Cell Sci. 116:17.
    2. Rikitake, Y. and Y. Takai (2008) Cell. Mol. Life Sci. 65:253.
    3. Sasisaka, T. et al. (2007) Curr. Opin. Cell Biol. 19:1.
    4. Aoki, J. et al. (1994) J. Biol. Chem. 269:8431.
    5. Takahashi, K. et al. (1999) J. Cell Biol. 145:539.
    6. Aoki, J. et al. (1997) Exp. Cell Res. 235:374.
    7. Lopez, M. et al. (1998) Blood 92:4602.
    8. Morrison, M.E. and V.R. Racaniello (1992) J. Virol. 66:2807.
    9. Struyf, F. et al. (2002) J. Virol. 76:12940.
    10. Delboy, M.G. et al. (2006) Virology J. 3:105.
    11. Irie, K. et al. (2004) Semin. Cell Dev. Biol. 15:643.
    12. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
    13. Satoh-Horikawa, K. et al. (2000) J. Biol. Chem. 275:10291.
    14. Nakanishi, H. and Y. Takai (2004) Biol. Chem. 385:885.
    15. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
    16. Pende, D. et al. (2006) Blood 107:2030.
  • Long Name:
    Poliovirus Receptor Related 2
  • Entrez Gene IDs:
    5819 (Human); 19294 (Mouse)
  • Alternate Names:
    CD112 antigen; CD112; Herpes virus entry mediator B; Herpesvirus entry mediator B; herpesvirus entry protein B; HVEB; HVEBpoliovirus receptor-like 2; MPH; nectin 2; Nectin2; Nectin-2; poliovirus receptor-related 2 (herpesvirus entry mediator B); poliovirus receptor-related protein 2; PRR2; PRR2nectin-2; PVRL2; PVRR2poliovirus receptor related 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity.
    Authors: Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N
    Leukemia, 2014;28(6):1316-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Rat IgG2A Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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