Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma -chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1‑7).
Mouse Netrin‑1 Biotinylated Antibody
R&D Systems | Catalog # BAF1109
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Val22-Ala603
Accession # AAC52971
Specificity
Clonality
Host
Isotype
Applications for Mouse Netrin‑1 Biotinylated Antibody
Western Blot
Sample: Recombinant Mouse Netrin‑1 (Catalog # 1109-N1)
Reviewed Applications
Read 1 review rated 3 using BAF1109 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Netrin-1
References
- Puschel, A. (1999) Mech. Dev. 83:65.
- Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
- Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
- Madison, R. et al. (2000) Exp. Neurology 161:563.
- Srinivasan, K. et al. (2003) Dev. Cell 4:371.
- Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
- Corset, V. et al. (2000) Nature 407:747.
Alternate Names
Gene Symbol
UniProt
Additional Netrin-1 Products
Product Documents for Mouse Netrin‑1 Biotinylated Antibody
Certificate of Analysis
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Product Specific Notices for Mouse Netrin‑1 Biotinylated Antibody
This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.
U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.
For research use only
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Citations for Mouse Netrin‑1 Biotinylated Antibody
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Protocols
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- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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