Mouse Neuropilin-1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5994A
Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
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Citations (1)
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Mouse Neuropilin-1 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Neuropilin-1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant rat (rr) Neuropilin-1 is observed and no cross-reactivity with recombinant human (rh) Neuropilin-1, rhNeuropilin-2, or rrNeuropilin-2 is observed.
Source
Monoclonal Rat IgG2A Clone # 761705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neuropilin-1
Phe21-Pro856
Accession # P97333
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Neuropilin‑1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin-1 APC-conjugated Monoclonal Antibody (Catalog # FAB5994A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Neuropilin-1

Neuropilin-1 (Nrp-1), also known as CD304, is a 130-140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1-4). The full-length 923 amino acid (aa) mouse Nrp-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced (5). This form is potentially a soluble antagonist, based on results from human Nrp-1 splice variants (1, 6-8). The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp-1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). Nrp-1 and Nrp-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Nrp-1 binds Sema3A, and only Nrp-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF165 binding (1). Sema3A signaling can be blocked by VEGF165, which has higher affinity for Nrp-1 (12). Nrp-1 is preferentially expressed in developing or remodeling arteries (1, 2). Nrp-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).

References
  1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. 
  2. Gu, C. et al. (2003) Dev. Cell 5:45. 
  3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
  4. Soker, S. et al. (1998) Cell 92:735.
  5. Entrez accession #EDL11827
  6. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. 
  7. Cackowski, F.C. et al. (2004) Genomics 84:82. 
  8. Rossignol, M. et al. (2000) Genomics 70:211.
  9. Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
  10. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
  11. Yaron, A. et al. (2005) Neuron 45:513.
  12. Narazaki, M. and G. Tosato (2006) Blood 107:3892.
  13. Tordjman, R. et al. (2002) Nat. Immunol. 3477.
Entrez Gene IDs
8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names
BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NP1; NRP; NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R

Product Datasheets

Citation for Mouse Neuropilin-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expansion of Foxp3(+) T-cell populations by Candida albicans enhances both Th17-cell responses and fungal dissemination after intravenous challenge.
    Authors: Whibley N, Maccallum D, Vickers M, Zafreen S, Waldmann H, Hori S, Gaffen S, Gow N, Barker R, Hall A
    Eur J Immunol, 2014;44(4):1069-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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