Mouse Neuropilin‑1 PE‑conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Neuropilin-1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant rat (rr) Neuropilin-1 is observed and no cross-reactivity with recombinant human (rh) Neuropilin-1, rhNeuropilin-2, or rrNeuropilin-2 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 761705
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Neuropilin-1
    Phe21-Pro856
    Accession # P97333
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5994P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Neuropilin-1
Neuropilin-1 (Nrp-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis. The full-length 923 amino acid (aa) mouse Nrp-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1. The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced. This form is potentially a soluble antagonist, based on results from human Nrp-1 splice variants. The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp-1 a1a2. Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), plus the C-terminus of Sema3 bind the b1b2 region of Nrp-1. Nrp-1 and Nrp-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. Both neuropilins act as co-receptors with plexins, including plexins A1, A4, B1, and D1, to bind class III semaphorins, such as Sema 3A, 3C, 3D, 3E, and 3F, that often mediate axon repulsion. Both Nrp-1 and Nrp-2 serve as co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF165 binding. Sema3A signaling can be blocked by VEGF165, which has higher affinity for Npn-1. Nrp-1 is preferentially expressed in developing or remodeling arteries. Nrp-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation.
  • Entrez Gene IDs:
    8829 (Human); 18186 (Mouse); 246331 (Rat)
  • Alternate Names:
    BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; neuropilin-1; NRP1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 13
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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