Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
MELA20
Mouse Neutrophil Elastase/ELA2 Standard Curve
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Product Details
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Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
3.37 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse ELA2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Mouse Neutrophil Elastase/ELA2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure ELA2 in cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse ELA2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse ELA2. Results obtained using natural mouse ELA2 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ELA2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 104 254 735 96.9 238 709
Standard Deviation 1.28 3.64 15.7 9.39 17.4 43.6
CV% 1.2 1.4 2.1 9.7 7.3 6.1

Recovery

The recovery of mouse ELA2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 90-102
Cell Lysis Buffer (n=3) 96 91-97
EDTA Plasma (n=4) 108 95-125
Heparin Plasma (n=4) 108 101-118
Serum (n=4) 112 104-122

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse ELA2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse Neuotrphil Elastase/ELA2 Linearity

Scientific Data

Mouse Neutrophil Elastase/ELA2 Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Entrez Gene IDs:
1991 (Human); 50701 (Mouse)
Alternate Names:
Bone marrow serine protease; EC 3.4.21; EC 3.4.21.37; ELA2; ELA2granulocyte-derived elastase; ELANE; elastase 2, neutrophil; elastase, neutrophil expressed; Elastase-2; GE; HLEelastase-2; HNE; Human leukocyte elastase; Leukocyte Elastase; Medullasin; NE; Neutrophil Elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1

Citation for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Pseudomonas aeruginosa-induced nociceptor activation increases susceptibility to infection
    Authors: T Lin, D Quellier, J Lamb, T Voisin, P Baral, F Bock, A Schönberg, R Mirchev, G Pier, I Chiu, M Gadjeva
    PloS Pathogens, 2021-05-06;17(5):e1009557.
    Species: Mouse
    Sample Types: Tissue Homogenates

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Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
By Anonymous on 12/11/2021
Sample Tested: HUVEC human umbilical vein endothelial cells