Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.
Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
R&D Systems | Catalog # MELA20
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (13 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
3.37 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
The Quantikine® Mouse Neutrophil Elastase/ELA2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure ELA2 in cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse ELA2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse ELA2. Results obtained using natural mouse ELA2 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ELA2.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse ELA2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 104 | 254 | 735 | 96.9 | 238 | 709 |
| Standard Deviation | 1.28 | 3.64 | 15.7 | 9.39 | 17.4 | 43.6 |
| CV% | 1.2 | 1.4 | 2.1 | 9.7 | 7.3 | 6.1 |
Recovery for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
The recovery of mouse ELA2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 90-102 |
| Cell Lysis Buffer (n=3) | 96 | 91-97 |
| EDTA Plasma (n=4) | 108 | 95-125 |
| Heparin Plasma (n=4) | 108 | 101-118 |
| Serum (n=4) | 112 | 104-122 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse ELA2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
Mouse Neutrophil Elastase/ELA2 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Neutrophil Elastase/ELA2
Alternate Names
ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin
Gene Symbol
ELANE
Additional Neutrophil Elastase/ELA2 Products
Product Documents for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
For research use only
Related Research Areas
Citations for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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