Mouse NKp46/NCR1 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2225F-025
FAB2225F-100

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Detection of NKp46/NCR1 in Mouse Splenocytes by Flow Cytometry.
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Citations (3)
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Mouse NKp46/NCR1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) NKp46 and less than 1% cross-reactivity with rhNKp80 and rhNKp30 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1
Glu22-Asn255
Accession # Q8C567
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NKp46/NCR1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of NKp46/NCR1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2225F) and Mouse Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog # FAB8319A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

References
  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
  4. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
  5. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  6. Arnon, T. et al. (2004) Blood 103:664.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  8. Mandelboim, O. et al. (2001) Nature 409:1055.
Entrez Gene IDs
9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)
Alternate Names
CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

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Citations for Mouse NKp46/NCR1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Deregulation of type I IFN-dependent genes correlates with increased susceptibility to cytomegalovirus acute infection of dicer mutant mice.
    Authors: Ostermann E, Tuddenham L, Macquin C, Alsaleh G, Schreiber-Becker J, Tanguy M, Bahram S, Pfeffer S, Georgel P
    PLoS ONE, 2012-08-20;7(8):e43744.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
    Authors: Li Z, Burns AR, Miller SB, Smith CW
    FASEB J., 2011-04-25;25(8):2659-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Inhibition of p-STAT3 enhances IFN-alpha efficacy against metastatic melanoma in a murine model.
    Authors: Kong LY, Gelbard A, Wei J
    Clin. Cancer Res., 2010-04-13;16(9):2550-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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