Mouse NKp46/NCR1 Antibody

  (20 citations)     
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Product Details
Citations (20)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) NKp46 is observed and less than 1% cross-reactivity with rhNKp80 and rhNKp30 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1
    Glu22-Asn255
    Accession # Q8C567
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse NKp46/NCR1 Fc Chimera (Catalog # 2225-NK)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    3-15 µg/mL
    See below
  • Agonist Activity
    0.4-2.4 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Mouse NKp46/NCR1 Antibody Induces IFN-gamma Secretion in Activated Mouse NK Cells. Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML), in a dose-dependent manner, as measured using the Quantikine Mouse IFN-gamma ELISA Kit (Catalog # MIF00). The ED50 for this effect is typically
0.4-2.4 μg/mL.
Detection of NKp46/NCR1 in Mouse DX5/CD49b+ Splenocytes by Flow Cytometry. Mouse DX5/CD49b+ splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Immunohistochemistry
NKp46/NCR1 in Mouse Spleen. NKp46/NCR1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
    2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    3. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
    4. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
    5. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    6. Arnon, T. et al. (2004) Blood 103:664.
    7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
    8. Mandelboim, O. et al. (2001) Nature 409:1055.
  • Entrez Gene IDs:
    9437 (Human); 17086 (Mouse)
  • Alternate Names:
    CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. NKp46 Calibrates Tumoricidal Potential of Type 1 Innate Lymphocytes by Regulating TRAIL Expression
    Authors: G Turchinovi, S Ganter, A Bärenwaldt, D Finke
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. NK cell heparanase controls tumor invasion and immune surveillance
    Authors: EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo
    Sci Rep, 2016;6(0):30636.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  5. Independent control of natural killer cell responsiveness and homeostasis at steady-state by CD11c+ dendritic cells
    Sci Rep, 2016;6(0):37996.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  6. Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival
    Authors: W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  7. Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  8. IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-gamma.
    Authors: Guo Y, Luan L, Rabacal W, Bohannon J, Fensterheim B, Hernandez A, Sherwood E
    J Immunol, 2015;195(5):2353-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
    Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
    J Leukoc Biol, 2015;97(3):583-98.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  10. Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke.
    Authors: Mracsko E, Liesz A, Stojanovic A, Lou W, Osswald M, Zhou W, Karcher S, Winkler F, Martin-Villalba A, Cerwenka A, Veltkamp R
    J Neurosci, 2014;34(50):16784-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure.
    Authors: Stolberg V, Martin B, Mancuso P, Olszewski M, Freeman C, Curtis J, Chensue S
    Am J Pathol, 2014;184(2):454-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  12. BCMab1, a monoclonal antibody against aberrantly glycosylated integrin alpha3beta1, has potent antitumor activity of bladder cancer in vivo.
    Authors: Li C, Yang Z, Du Y, Tang H, Chen J, Hu D, Fan Z
    Clin Cancer Res, 2014;20(15):4001-13.
    Species: Mouse
    Sample Type:
  13. TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Authors: Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke C, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lepinasse F, Marvel J, Verhoeyen E, Cosset F, Peeper D, Smyth M, Londono-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec J, Stoppacciaro A, Leonetti C, Vivier E, Gilson E
    Nat Cell Biol, 2013;15(7):818-28.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  14. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
    Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
    PLoS Pathog., 2012;8(1):e1002481.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  15. Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
    Authors: Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
    J. Immunol., 2011;187(6):3096-103.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  16. Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
    Authors: Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(21):9759-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  17. Distinct phenotype and function of NK cells in the pancreas of nonobese diabetic mice.
    Authors: Brauner H, Elemans M, Lemos S, Broberger C, Holmberg D, Flodstrom-Tullberg M, Karre K, Hoglund P
    J. Immunol., 2010;184(5):2272-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  18. Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.
    Authors: Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M
    J. Immunol., 2009;183(10):6489-99.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  19. Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells.
    Authors: Kang SJ, Liang HE, Reizis B, Locksley RM
    Immunity, 2008;29(5):819-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  20. CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells.
    Authors: Vosshenrich CA, Lesjean-Pottier S, Hasan M, Richard-Le Goff O, Corcuff E, Mandelboim O, Di Santo JP
    J. Exp. Med., 2007;204(11):2569-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all Citations

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