Mouse Nogo Receptor/NgR Antibody

Catalog # Availability Size / Price Qty
AF1440
AF1440-SP
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (1)

Mouse Nogo Receptor/NgR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Nogo Receptor in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant human (rh) NgR is observed and less than 1% cross-reactivity with rhNgRH1 and rhNgRH2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NgR
Cys27-Ser447
Accession # Q99PI8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Nogo Receptor/NgR Fc Chimera (Catalog # 1440-NG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo Receptor/NgR

Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid (aa) residue precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTR  then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).

References
  1. Fournier, A.E. et al. (2001) Nature 409:341.
  2. GrandPre, T. et al. (2002) Nature 417:547.
  3. Wang, K.C. et al. (2002) Nature 420:74.
  4. Barton, W.A. et al. (2003) EMBO Journal 22:3291.
  5. Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
  6. Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.
Long Name
Nogo-66 Receptor/Reticulon 4 Receptor
Entrez Gene IDs
65078 (Human); 65079 (Mouse)
Alternate Names
NgR; NgR1; Nogo R; Nogo receptor; Nogo-66 receptor; NogoR; NOGORUNQ330/PRO526; reticulon 4 receptor; reticulon-4 receptor; RTN4R

Product Datasheets

Citations for Mouse Nogo Receptor/NgR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling
    Authors: S Fricke, K Metzdorf, M Ohm, S Haak, M Heine, M Korte, M Zagrebelsk
    Cell Rep, 2019;29(3):671-684.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Nogo receptor 1 is expressed by nearly all retinal ganglion cellsPV expression in Figure 3
    Authors: AM Solomon, T Westbrook, GD Field, AW McGee
    PLoS ONE, 2018;13(5):e0196565.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor
    Authors: Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. LOTUS overexpression accelerates neuronal plasticity after focal brain ischemia in mice
    Authors: H Takase, Y Kurihara, TA Yokoyama, N Kawahara, K Takei
    PLoS ONE, 2017;12(9):e0184258.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Regulation of axonal regeneration by the level of function of the endogenous Nogo receptor antagonist LOTUS
    Authors: T Hirokawa, Y Zou, Y Kurihara, Z Jiang, Y Sakakibara, H Ito, K Funakoshi, N Kawahara, Y Goshima, SM Strittmatt, K Takei
    Sci Rep, 2017;7(1):12119.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve.
    Authors: Vajda F, Jordi N, Dalkara D, Joly S, Christ F, Tews B, Schwab M, Pernet V
    Cell Death Differ, 2015;22(2):323-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Plasticity of binocularity and visual acuity are differentially limited by nogo receptor.
    Authors: Stephany C, Chan L, Parivash S, Dorton H, Piechowicz M, Qiu S, McGee A
    J Neurosci, 2014;34(35):11631-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. LGI1 is a Nogo receptor 1 ligand that antagonizes myelin-based growth inhibition.
    Authors: Thomas R, Favell K, Morante-Redolat J, Pool M, Kent C, Wright M, Daignault K, Ferraro GB, Montcalm S, Durocher Y, Fournier A, Perez-Tur J, Barker PA
    J. Neurosci., 2010;30(19):6607-12.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Soluble Nogo receptor down-regulates expression of neuronal Nogo-A to enhance axonal regeneration.
    Authors: Peng X, Zhou Z, Hu J, Fink DJ, Mata M
    J. Biol. Chem., 2010;285(4):2783-95.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Localization of an axon growth inhibitory molecule Nogo and its receptor in the spinal cord of mouse embryos.
    Authors: Wang J, Wang L, Zhao H, Chan SO
    Brain Res., 2010;1306(0):8-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Reassessment of corticospinal tract regeneration in Nogo-deficient mice.
    Authors: Lee JK, Chan AF, Luu SM, Zhu Y, Ho C, Tessier-Lavigne M, Zheng B
    J. Neurosci., 2009;29(27):8649-54.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength.
    Authors: Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ
    J. Neurosci., 2008;28(11):2753-65.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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Mouse Nogo Receptor/NgR Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes Species: Mouse