Mouse Nogo Receptor/NgR Antibody
Mouse Nogo Receptor/NgR Antibody Summary
Cys27-Ser447
Accession # Q99PI8
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo Receptor/NgR
Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid (aa) residue precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTR then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).
- Fournier, A.E. et al. (2001) Nature 409:341.
- GrandPre, T. et al. (2002) Nature 417:547.
- Wang, K.C. et al. (2002) Nature 420:74.
- Barton, W.A. et al. (2003) EMBO Journal 22:3291.
- Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
- Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.
Product Datasheets
Citations for Mouse Nogo Receptor/NgR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling
Authors: S Fricke, K Metzdorf, M Ohm, S Haak, M Heine, M Korte, M Zagrebelsk
Cell Rep, 2019;29(3):671-684.e6.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Nogo receptor 1 is expressed by nearly all retinal ganglion cellsPV expression in Figure 3
Authors: AM Solomon, T Westbrook, GD Field, AW McGee
PLoS ONE, 2018;13(5):e0196565.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor
Authors: Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei
J. Neurosci., 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
LOTUS overexpression accelerates neuronal plasticity after focal brain ischemia in mice
Authors: H Takase, Y Kurihara, TA Yokoyama, N Kawahara, K Takei
PLoS ONE, 2017;12(9):e0184258.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Regulation of axonal regeneration by the level of function of the endogenous Nogo receptor antagonist LOTUS
Authors: T Hirokawa, Y Zou, Y Kurihara, Z Jiang, Y Sakakibara, H Ito, K Funakoshi, N Kawahara, Y Goshima, SM Strittmatt, K Takei
Sci Rep, 2017;7(1):12119.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve.
Authors: Vajda F, Jordi N, Dalkara D, Joly S, Christ F, Tews B, Schwab M, Pernet V
Cell Death Differ, 2015;22(2):323-35.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Plasticity of binocularity and visual acuity are differentially limited by nogo receptor.
Authors: Stephany C, Chan L, Parivash S, Dorton H, Piechowicz M, Qiu S, McGee A
J Neurosci, 2014;34(35):11631-40.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
LGI1 is a Nogo receptor 1 ligand that antagonizes myelin-based growth inhibition.
Authors: Thomas R, Favell K, Morante-Redolat J, Pool M, Kent C, Wright M, Daignault K, Ferraro GB, Montcalm S, Durocher Y, Fournier A, Perez-Tur J, Barker PA
J. Neurosci., 2010;30(19):6607-12.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Soluble Nogo receptor down-regulates expression of neuronal Nogo-A to enhance axonal regeneration.
Authors: Peng X, Zhou Z, Hu J, Fink DJ, Mata M
J. Biol. Chem., 2010;285(4):2783-95.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Localization of an axon growth inhibitory molecule Nogo and its receptor in the spinal cord of mouse embryos.
Authors: Wang J, Wang L, Zhao H, Chan SO
Brain Res., 2010;1306(0):8-17.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Reassessment of corticospinal tract regeneration in Nogo-deficient mice.
Authors: Lee JK, Chan AF, Luu SM, Zhu Y, Ho C, Tessier-Lavigne M, Zheng B
J. Neurosci., 2009;29(27):8649-54.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength.
Authors: Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ
J. Neurosci., 2008;28(11):2753-65.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
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