Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid (aa) residue precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTR then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).
Mouse Nogo Receptor/NgR Antibody
R&D Systems | Catalog # AF1440
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NgR
Cys27-Ser447
Accession # Q99PI8
Cys27-Ser447
Accession # Q99PI8
Specificity
Detects mouse Nogo Receptor in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant human (rh) NgR is observed and less than 1% cross-reactivity with rhNgRH1 and rhNgRH2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Nogo Receptor/NgR Antibody
Detection of Mouse Nogo Receptor/NgR by Western Blot
NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Nogo Receptor/NgR by Western Blot
NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Nogo Receptor/NgR by Western Blot
NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse Nogo Receptor/NgR Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Nogo Receptor/NgR Fc Chimera (Catalog # 1440-NG)
Sample: Recombinant Mouse Nogo Receptor/NgR Fc Chimera (Catalog # 1440-NG)
Reviewed Applications
Read 2 reviews rated 3.5 using AF1440 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Nogo Receptor/NgR
References
- Fournier, A.E. et al. (2001) Nature 409:341.
- GrandPre, T. et al. (2002) Nature 417:547.
- Wang, K.C. et al. (2002) Nature 420:74.
- Barton, W.A. et al. (2003) EMBO Journal 22:3291.
- Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
- Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.
Long Name
Nogo-66 Receptor/Reticulon 4 Receptor
Alternate Names
NgR1, NogoR, RTN4R
Gene Symbol
RTN4R
UniProt
Additional Nogo Receptor/NgR Products
Product Documents for Mouse Nogo Receptor/NgR Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Nogo Receptor/NgR Antibody
For research use only
Related Research Areas
Citations for Mouse Nogo Receptor/NgR Antibody
Powered by Bioz
Customer Reviews for Mouse Nogo Receptor/NgR Antibody (2)
3.5 out of 5
2 Customer Ratings
Have you used Mouse Nogo Receptor/NgR Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
2 of
2 reviews
Showing All
Filter By:
-
Application: Flow CytometrySample Tested: Adult splenocytesSpecies: MouseVerified Customer | Posted 08/24/2017
-
Application: ImmunofluorescenceSample Tested: Mouse neurons DIV12Species: MouseVerified Customer | Posted 12/19/2014
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...