Mouse Nogo Receptor/NgR Antibody

Catalog # Availability Size / Price Qty
AF1440
AF1440-SP

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Detection of Mouse Nogo Receptor/NgR by Western Blot
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Product Details
Citations (17)
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Mouse Nogo Receptor/NgR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Nogo Receptor in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant human (rh) NgR is observed and less than 1% cross-reactivity with rhNgRH1 and rhNgRH2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NgR
Cys27-Ser447
Accession # Q99PI8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Nogo Receptor/NgR Fc Chimera (Catalog # 1440-NG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Nogo Receptor/NgR by Western Blot View Larger

Detection of Mouse Nogo Receptor/NgR by Western Blot NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Nogo Receptor/NgR by Western Blot View Larger

Detection of Mouse Nogo Receptor/NgR by Western Blot NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Nogo Receptor/NgR by Western Blot View Larger

Detection of Mouse Nogo Receptor/NgR by Western Blot NgR1 and LGI1 regulate synaptic proteins in cortical neurons in vitro.A, Twiss filter schematic showing culture system to coculture hippocampal neurons with astrocytes and separate neuronal processes from cell bodies. Hippocampal neurons seeded on filters with a pore size 1 µm that cell bodies will not pass through. Axons and dendrites grow on the filter tops and extend down onto the filter bottom. Astrocytes are seeded on the bottom of the well to provide growth factors. B, Time course of lysates from hippocampal neurons grown on filters suspended over an astrocyte feeder layer for the times indicated. The first lane in the left panel labeled E16 is a sample of hippocampal neurons lysed directly after dissociated before plating. Lysates from filter tops including cell bodies and processes are on the left. Lysates of the filter bottoms containing axons and dendrites but no cell bodies are on the right. Antibodies used to probe the lysates are indicated on the right. Histone-3 (H3), a structural protein found in chromatin and present only in the nucleus is detected only in the cell body lysates. C, Lysates from filter bottoms containing axons and dendrite but not cell bodies from LGI1+/+ and LGI1-/- littermates of cortical cultures grown for the indicated number of DIV. D, Quantification of PSD95 levels relative to actin levels and normalized to WT controls in LGI1 samples at 12, 15, and 18 DIV, n = 3 separate experiments. E, Western blottings of lysates from filter bottoms of NgR1+/+ and NgR1-/- cortical cultures harvested at 12, 15, or 18 DIV synaptic markers, Syn and PSD95. Actin and Tuj1 are loading controls. F, Quantification of PSD95 relative to actin levels and normalized to WT controls in NgR1, n = 4 separate experiments. Significant differences are indicated on the graphs analysis was performed by two-way ANOVA with Bonferroni post hoc tests, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30225353), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo Receptor/NgR

Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid (aa) residue precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTR  then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).

References
  1. Fournier, A.E. et al. (2001) Nature 409:341.
  2. GrandPre, T. et al. (2002) Nature 417:547.
  3. Wang, K.C. et al. (2002) Nature 420:74.
  4. Barton, W.A. et al. (2003) EMBO Journal 22:3291.
  5. Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
  6. Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.
Long Name
Nogo-66 Receptor/Reticulon 4 Receptor
Entrez Gene IDs
65078 (Human); 65079 (Mouse)
Alternate Names
NgR; NgR1; Nogo R; Nogo receptor; Nogo-66 receptor; NogoR; NOGORUNQ330/PRO526; reticulon 4 receptor; reticulon-4 receptor; RTN4R

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Citations for Mouse Nogo Receptor/NgR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling
    Authors: S Fricke, K Metzdorf, M Ohm, S Haak, M Heine, M Korte, M Zagrebelsk
    Cell Rep, 2019-10-15;29(3):671-684.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. The Nogo Receptor Ligand LGI1 Regulates Synapse Number and Synaptic Activity in Hippocampal and Cortical Neurons
    Authors: Rhalena A. Thomas, Julien Gibon, Carol X. Q. Chen, Sabrina Chierzi, Vincent G. Soubannier, Stephanie Baulac et al.
    eNeuro
  3. Nogo receptor 1 is expressed by nearly all retinal ganglion cellsPV expression in Figure 3
    Authors: AM Solomon, T Westbrook, GD Field, AW McGee
    PLoS ONE, 2018-05-16;13(5):e0196565.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. The nociceptin receptor inhibits axonal regeneration and recovery from spinal cord injury
    Authors: Yuichi Sekine, Chad S. Siegel, Tomoko Sekine-Konno, William B. J. Cafferty, Stephen M. Strittmatter
    Science Signaling
  5. The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor
    Authors: Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei
    J. Neurosci., 2018-02-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Amyloid Beta Peptides Block New Synapse Assembly by Nogo Receptor-Mediated Inhibition of T-Type Calcium Channels
    Authors: Yanjun Zhao, Sivaprakash Sivaji, Michael C. Chiang, Haadi Ali, Monica Zukowski, Sareen Ali et al.
    Neuron
  7. Regulation of axonal regeneration by the level of function of the endogenous Nogo receptor antagonist LOTUS
    Authors: T Hirokawa, Y Zou, Y Kurihara, Z Jiang, Y Sakakibara, H Ito, K Funakoshi, N Kawahara, Y Goshima, SM Strittmatt, K Takei
    Sci Rep, 2017-09-21;7(1):12119.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. LOTUS overexpression accelerates neuronal plasticity after focal brain ischemia in mice
    Authors: H Takase, Y Kurihara, TA Yokoyama, N Kawahara, K Takei
    PLoS ONE, 2017-09-07;12(9):e0184258.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Erasure of fear memories is prevented by Nogo Receptor 1 in adulthood
    Authors: Sarah M. Bhagat, Santino S. Butler, Jane R. Taylor, Bruce S. McEwen, Stephen M. Strittmatter
    Molecular Psychiatry
  10. Human NgR-Fc Decoy Protein via Lumbar Intrathecal Bolus Administration Enhances Recovery from Rat Spinal Cord Contusion
    Authors: Xingxing Wang, Kazim Yigitkanli, Chang-Yeon Kim, Tomoko Sekine-Konno, Dana Wirak, Eric Frieden et al.
    Journal of Neurotrauma
  11. Cell type-specific Nogo-A gene ablation promotes axonal regeneration in the injured adult optic nerve.
    Authors: Vajda F, Jordi N, Dalkara D, Joly S, Christ F, Tews B, Schwab M, Pernet V
    Cell Death Differ, 2014-09-26;22(2):323-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Plasticity of binocularity and visual acuity are differentially limited by nogo receptor.
    Authors: Stephany C, Chan L, Parivash S, Dorton H, Piechowicz M, Qiu S, McGee A
    J Neurosci, 2014-08-27;34(35):11631-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. LGI1 is a Nogo receptor 1 ligand that antagonizes myelin-based growth inhibition.
    Authors: Thomas R, Favell K, Morante-Redolat J, Pool M, Kent C, Wright M, Daignault K, Ferraro GB, Montcalm S, Durocher Y, Fournier A, Perez-Tur J, Barker PA
    J. Neurosci., 2010-05-12;30(19):6607-12.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Soluble Nogo receptor down-regulates expression of neuronal Nogo-A to enhance axonal regeneration.
    Authors: Peng X, Zhou Z, Hu J, Fink DJ, Mata M
    J. Biol. Chem., 2009-11-09;285(4):2783-95.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Localization of an axon growth inhibitory molecule Nogo and its receptor in the spinal cord of mouse embryos.
    Authors: Wang J, Wang L, Zhao H, Chan SO
    Brain Res., 2009-10-13;1306(0):8-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Reassessment of corticospinal tract regeneration in Nogo-deficient mice.
    Authors: Lee JK, Chan AF, Luu SM, Zhu Y, Ho C, Tessier-Lavigne M, Zheng B
    J. Neurosci., 2009-07-08;29(27):8649-54.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength.
    Authors: Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ
    J. Neurosci., 2008-03-12;28(11):2753-65.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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Mouse Nogo Receptor/NgR Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes Species: Mouse