Mouse Nope/IGDCC4 Antibody

Catalog # Availability Size / Price Qty
AF1394
AF1394-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Mouse Nope/IGDCC4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Nope/IGDCC4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity  with recombinant mouse (rm) DCC, rmNeogenin, and rmPUNC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Nope/IGDCC4
Gly22-His953
Accession # Q9EQS9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Nope/IGDCC4 Fc Chimera (Catalog # 1394-NP)

Mouse Nope/IGDCC4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Nope/IGDCC4 Biotinylated Antibody (Catalog # BAF1394)

Standard: Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF (Catalog # 1394-NP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nope/IGDCC4

Mouse Nope (Neighbor Of Punc E11) was discovered as a gene proximal to the Punc gene on chromosome 9 (1). Punc and Nope are distant members of a subgroup of the immunoglobulin (Ig) superfamily, which include DCC (Deleted in Colorectal Cancer) (2), Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (rat UNC5H1 and H2, mouse UNC5H2 and H3, and human UNC5H3 and H4) (3), Drosophila Frazzled (4), vertebrate Neogenin (5), and mouse Nope and mouse Punc. Members of this subgroup of the Ig superfamily are type I transmembrane proteins with four Ig domains in their extracellular regions.

Mouse Nope consists of a 21 amino acid (aa) signal peptide, a 933 aa extracellular domain (including four Ig domains, five fibronectin-type III (FnIII) repeats), a 24 aa transmembrane segment, and a 274 aa cytoplasmic domain (1). The extracellular domain of mouse Nope shares 45% aa sequence similarity with mouse Punc. However, the cytoplasmic domains of mouse Nope and mouse Punc do not share aa sequence similarity. Compared to other members of the subgroup of the Ig superfamily, mouse Nope extracellular domain shares 25.8% and 25.2% aa similarity with mouse DCC and mouse Neogenin, respectively. Mouse and human Nope share 90.8% aa sequence similarity. Mouse Nope is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between Nope and the guidance receptor of the DCC family suggest that Nope may have similar functions as the DCC family (6‑8).

References
  1. Salbaum, J.M. and C. Kappen (2000) Genomics 64:15.
  2. Fearon et al. (1990) Science 247:49.
  3. Keino-Masu et al. (1996) Cell 87:175.
  4. Hong, K. et al. (1999) Cell 97:927.
  5. Leonardo, E.D. et al. (1997) Nature 386:833.
  6. Kolodziej et al. (1996) Cell 87:197.
  7. Meyerhardt (1997) Oncogene 14:1129.
  8. Vielmetter et al. (1997) Genomics 41:414.
Long Name
Neighbor of Punc E11/Immunoglobulin Superfamily, DCC Subclass, Member 4
Entrez Gene IDs
57722 (Human); 56741 (Mouse)
Alternate Names
DDM36; hDDM36; IGDCC4; immunoglobulin superfamily DCC subclass member 4; immunoglobulin superfamily, DCC subclass, member 4; KIAA1628; likely ortholog of mouse neighbor of Punc E11; LOC57722; Neighbor of punc e11; Nope; NOPEFLJ42051; Protein DDM36

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Citations for Mouse Nope/IGDCC4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Depolarized Hepatocytes Express the Stem/Progenitor Cell Marker Neighbor of Punc E11 After Bile Duct Ligation in Mice
    Authors: A Bowe, S Zweerink, V Mück, V Kondylis, S Schulte, T Goeser, D Nierhoff
    J. Histochem. Cytochem., 2018-04-06;0(0):2215541876823.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  2. New cell surface markers for murine fetal hepatic stem cells identified through high density complementary DNA microarrays.
    Authors: Nierhoff, Dirk, Levoci, Lauretta, Schulte, Sigrid, Goeser, Tobias, Rogler, Leslie E, Shafritz, David A
    Hepatology, 2007-08-01;46(2):535-47.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr, Western Blot

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