Mouse Osteoprotegerin/TNFRSF11B Biotinylated Antibody BAF459: R&D Systems

Mouse Osteoprotegerin/TNFRSF11B Biotinylated Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Osteoprotegerin/TNFRSF11B in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse RANK is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Osteoprotegerin/TNFRSF11B
    Gln21-Leu401 (Gln138Arg)
    Accession # Q6PI12
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera (Catalog # 459-MO)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse Osteoprotegerin/TNFRSF11B Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse Osteoprotegerin/TNFRSF11B Antibody (Catalog # MAB4591)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Osteoprotegerin/TNFRSF11B Biotinylated Antibody (Catalog # BAF459)
  • ELISA Standard
     
    Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera, CF (Catalog # 459-MO)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Osteoprotegerin/TNFRSF11B in Mouse Intestine. Osteoprotegerin/TNFRSF11B was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF459) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell‑association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The carboxy-terminal of OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor‑related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF-kappaB ligand) and TRAIL (TNF-related apoptosis‑inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1‑4 (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.

 

  • References:
    1. Lacey, D.L. et al. (1998) Cell 93:165.
    2. Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363.
    3. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597.
  • Entrez Gene IDs:
    4982 (Human); 18383 (Mouse)
  • Alternate Names:
    OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Osteoprotegerin-Mediated Homeostasis of Rank+ Thymic Epithelial Cells Does Not Limit Foxp3+ Regulatory T Cell Development.
    Authors: McCarthy N, Cowan J, Nakamura K, Bacon A, Baik S, White A, Parnell S, Jenkinson E, Jenkinson W, Anderson G
    J Immunol, 2015;195(6):2675-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice.
    Authors: Shimamura M, Nakagami H, Osako M, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R
    Proc Natl Acad Sci U S A, 2014;111(22):8191-6.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fluorescence
  3. Cytokine-induced osteoprotegerin expression protects pancreatic beta cells through p38 mitogen-activated protein kinase signalling against cell death.
    Authors: Schrader J, Rennekamp W, Niebergall U, Schoppet M, Jahr H, Brendel MD, Horsch D, Hofbauer LC
    Diabetologia, 2007;50(6):1243-7.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  4. Therapeutic relevance of osteoprotegerin gene therapy in osteosarcoma: blockade of the vicious cycle between tumor cell proliferation and bone resorption.
    Authors: Lamoureux F, Richard P, Wittrant Y, Battaglia S, Pilet P, Trichet V, Blanchard F, Gouin F, Pitard B, Heymann D, Redini F
    Cancer Res., 2007;67(15):7308-18.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Serotonin and fluoxetine modulate bone cell function in vitro.
    Authors: Gustafsson BI, Thommesen L, Stunes AK, Tommeras K, Westbroek I, Waldum HL, Slordahl K, Tamburstuen MV, Reseland JE, Syversen U
    J. Cell. Biochem., 2006;98(1):139-51.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  6. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Biotinylated Control

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