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OR

Mouse OX40/TNFRSF4 PE‑conjugated Antibody

  • Specificity
    Detects mouse OX40/TNFRSF4 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant mouse (rm) EDAR, rm4-BB, rmCD27, rmDR3, rmGITR, rmNGF R, rmCD30, rmCD40, rmFas, rmOPG, rmRANK, rmTNF RI, and rmTNF RII is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse OX40/TNFRSF4
    Val20-Pro211
    Accession # P47741
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of OX40/TNFRSF4 in Mouse Splenocytes by Flow Cytometry. Activated-mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Goat Anti-Mouse OX40/TNFRSF4 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1256P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: OX40/TNFRSF4

OX40, also known as CD134, was originally identified as an activated rat CD4+ T cell-surface antigen that is recognized by the monoclonal antibody MRC OX40. It is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF4. Mouse OX40 cDNA encodes a 256 amino acid (aa) residues type I transmembrane precursor protein with a putative 19 aa signal peptide, a 192 aa extracellular domain containing 4 TNFR-cysteine rich repeats, a 25 aa transmembrane domain and a 36 aa cytoplasmic region. A naturally occurring soluble OX40 has also been identified in human serum. Mouse OX40 shares approximately 63% and 90% aa sequence identity with its human and rat counterparts, respectively. OX40 is a T cell activation antigen that is expressed primarily on activated CD4+ T cells, but is also expressed on activated human and mouse CD8+ T cells. The ligand of OX40 is OX40 Ligand (OX40L), also known as gp34, a type II transmembrane glycoprotein belonging to the TNF superfamily. OX40L is expressed on activated B cells, T cells, dendritic cells and endothelial cells. Ligation of OX40 on T cells by OX40L or an agonistic antibody can promote clonal expansion, long-term T cell survival, and enhance memory T cell development. In vivo, blockade of OX40/OX40L interaction has been useful for treating autoimmune disease and graft-versus-host disease in animal models. Activation of OX40 has also been utilized to enhance the potency of vaccines and augment anti-tumor immunity (1-9).

  • References:
    1. Paterson, D.J. et al. (1987) Mol. Immunol. 24:1281.
    2. Mallett, S. et al. (1990) EMBO J. 9:1063.
    3. Calderhead, D.M. et al. (1993) J. Immunol. 151:5261.
    4. Baum, P.R. et al. (1994) EMBO J. 13:3992.
    5. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
    6. Bansal-Pakala, P. et al. (2001) Nature Medicine 7:907.
    7. Rogers, P.R. et al. (2001) Immunity 15:445.
    8. Taylor, L. and H.J. Schwarz (2001) Immunol. Methods 255:67.
    9. Weinberg, A.D. (2002) Trends in Immunol. 23:102.
  • Entrez Gene IDs:
    25572 (Human); 22163 (Human); 7293 (Human);
  • Alternate Names:
    ACT-135; CD134; TNFRSF4; ACT35 antigen; ACT35ATC35 antigen; CD134; CD134 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; TXGP1LOX40 antigen
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