Mouse OX40/TNFRSF4 Antibody

Catalog # Availability Size / Price Qty
AF1256
AF1256-SP
Product Details
Citations (2)
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Mouse OX40/TNFRSF4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse OX40/TNFRSF4 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant mouse (rm) EDAR, rm4-BB, rmCD27, rmDR3, rmGITR, rmNGF R, rmCD30, rmCD40, rmFas, rmOPG, rmRANK, rmTNF RI, and rmTNF RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse OX40/TNFRSF4
Val20-Pro211
Accession # P47741
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse OX40/TNFRSF4 Fc Chimera (Catalog # 1256-OX)
Agonist Activity
Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3 [Saoulli, K. et al. (1998) J. Exp. Med. 187(11):1849; Cannons, J. et al. (2001) J. Immunol. 167:1313.].
The ED50 for this effect is typically 0.4 - 1.2 μg/mL.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OX40

OX40, also known as CD134, was originally identified as an activated rat CD4+ T cell-surface antigen that is recognized by the monoclonal antibody MRC OX40. It is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF4. Mouse OX40 cDNA encodes a 256 amino acid (aa) residues type I transmembrane precursor protein with a putative 19 aa signal peptide, a 192 aa extracellular domain containing 4 TNFR-cysteine rich repeats, a 25 aa transmembrane domain and a 36 aa cytoplasmic region. A naturally occurring soluble OX40 has also been identified in human serum. Mouse OX40 shares approximately 63% and 90% aa sequence identity with its human and rat counterparts, respectively. OX40 is a T cell activation antigen that is expressed primarily on activated CD4+ T cells, but is also expressed on activated human and mouse CD8+ T cells. The ligand of OX40 is OX40 ligand (OX40L), also known as gp34, a type II transmembrane glycoprotein belonging to the TNF superfamily. OX40L is expressed on activated B cells, T cells, dendritic cells and endothelial cells. Ligation of OX40 on T cells by OX40L or an agonistic antibody can promote clonal expansion, long-term T cell survival, and enhance memory T cell development. In vivo, blockade of OX40/OX40L interaction has been useful for treating autoimmune disease and graft-versus-host disease in animal models. Activation of OX40 has also been utilized to enhance the potency of vaccines and augment anti-tumor immunity (1-9).

References
  1. Paterson, D.J. et al. (1987) Mol. Immunol. 24:1281.
  2. Mallett, S. et al. (1990) EMBO J. 9:1063.
  3. Calderhead, D.M. et al. (1993) J. Immunol. 151:5261.
  4. Baum, P.R. et al. (1994) EMBO J. 13:3992.
  5. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
  6. Bansal-Pakala, P. et al. (2001) Nature Medicine 7:907.
  7. Rogers, P.R. et al. (2001) Immunity 15:445.
  8. Taylor, L. and H.J. Schwarz (2001) Immunol. Methods 255:67.
  9. Weinberg, A.D. (2002) Trends in Immunol. 23:102.
Entrez Gene IDs
7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)
Alternate Names
ACT-135; ACT35 antigen; ACT35ATC35 antigen; CD134 antigen; CD134; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40; OX40L receptor; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; TNFRSF4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen

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Citations for Mouse OX40/TNFRSF4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Type 2 innate lymphoid cells drive CD4+ Th2 cell responses.
    Authors: Mirchandani, Ananda S, Besnard, Anne-Gae, Yip, Edwin, Scott, Charlott, Bain, Calum C, Cerovic, Vuk, Salmond, Robert J, Liew, Foo Y
    J Immunol, 2014-01-27;192(5):2442-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. OX40 complexes with phosphoinositide 3-kinase and protein kinase B (PKB) to augment TCR-dependent PKB signaling.
    Authors: So T, Choi H, Croft M
    J. Immunol., 2011-02-02;186(6):3547-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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