Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 261845
View all PCPE-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PCPE-1
Gln25-Ala468
Accession # Q61398

Specificity

Detects mouse PCPE-1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human PCPE-1 is observed. In Western blots, no cross-reactivity with recombinant human PCPE-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse PCPE-1 Antibody (261845)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse PCPE‑1, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Mouse PCPE‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PCPE-1

Procollagen C‑endopeptidase enhancers, known as PCPEs or PCOLCEs, are secreted extracellular matrix glycoproteins that consist of two CUB domains and one NTR domain. They are known to stimulate enzymatic cleavage of procollagens I‑III by the BMP-1/tolloid family of metalloproteases, also known as procollagen C‑proteinases (1). PCPE‑1 is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin (2). Although BMP-1/tolloid proteinases are involved in processing of multiple extracellular proteins, the enhancer activity of PCPE-1 is specific to procollagens since it has no effect on BMP‑1/tolloid cleavage of other substrates (3). It is thought that PCPE-1 enhances cleavage of procollagens by binding to the substrate, inducing a conformation change in the substrate (3), although interaction between PCPE-1 and full-length BMP‑1/tolloid proteinases has also been reported (4).

References

  1. Steiglitz, B.M. et al. (2002) J. Biol. Chem. 277:49820.
  2. Kessler, E. et al. (1990) Biochem. Biophys. Res. Commun. 173:81.
  3. Moali, C. et al. (2005) J. Biol. Chem. 280:24188.
  4. Ge, G. et al. (2006) J. Biol. Chem. 281:10786.

Long Name

Procollagen C-Proteinase Enhancer-1

Alternate Names

PCOLCE, PCPE1

Entrez Gene IDs

5118 (Human); 18542 (Mouse)

Gene Symbol

PCOLCE

UniProt

Q61398

Additional PCPE-1 Products

Product Documents for Mouse PCPE-1 Antibody (261845)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse PCPE-1 Antibody (261845)

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Mouse PCPE-1 Antibody (261845)

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