Mouse Pentraxin 2/SAP Antibody

Catalog # Availability Size / Price Qty
MAB2558
MAB2558-SP
Product Details
Citations (2)
FAQs
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Mouse Pentraxin 2/SAP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Pentraxin 2/SAP in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant rat Pentraxin 2/SAP is observed. In Western blots, 100% cross-reactivity with recombinant human (rh) Pentraxin 2/SAP is observed, but no cross-reactivity with rhPentraxin 2/SAP in direct ELISAs is observed.
Source
Monoclonal Rat IgG2A Clone # 300103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Pentraxin 2/SAP
Gln21-Asp224
Accession # P12246
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Pentraxin 2/SAP (Catalog # 2558-SA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pentraxin 2/SAP

Pentraxin 2 (also known as Serum Amyoid P Component or SAP) is a secreted glycoprotein that is a universal non-fibrillar component of amyloid deposits. Amyloid is an abnormal extracellular deposit of insoluble protein fibrils that can lead to tissue damage and disease (1‑3). Pentraxin 2 belongs to the pentraxin (pentaxin) family, whose members have a characteristic pentagonal discoid arrangement of five non-covalently bound subunits (4). Pentraxin domains contain the consensus sequence, HxCx(S/T)WxS (x = any amino acid), a lectin fold, and two calcium-binding sites (1). They bind to a variety of unrelated molecules in a calcium-dependent lectin-like manner (1, 4, 5). Pentraxin 2 and C-reactive protein (CRP) are members of the classical or short pentraxin subfamily and share 46% amino acid (aa) identity (1). Mouse Pentraxin 2 is the major acute-phase protein whose expression is dependent on complement activation, IL-6 and/or IL-1 beta, while in humans, CRP is the major acute-phase protein (2, 5, 9). Both are produced and secreted by liver hepatocytes and circulate in plasma. The 204 aa mature mouse Pentraxin 2 shares 79% aa identity with rat Pentraxin 2 and 63‑68% aa identity with human, guinea pig, golden hamster, porcine, and bovine Pentraxin 2 (2, 5). Amyloid deposits containing Pentraxin 2 are implicated in a diverse range of diseases including Alzheimer’s, prion diseases, type 2 diabetes and various systemic amyloidoses (3, 6, 7). Pentraxin 2 regulates the solubility of amyloid fibrils and protects them from degradation. In addition to its pathogenic role, Pentraxin 2 also has an important physiological function in innate immunity (8). It is an opsonin that interacts with all three types of human Fc gamma receptors that mediate neutrophil phagocytosis (8). Pentraxin 2 has been proposed to bind and sequester a variety of ligands including auto-antigens, apoptotic cells, chromatin, DNA, and micro-organisms (1‑3). Pentraxin 2 is also a normal component of basement membranes (1).

References
  1. Garlanda, C. et al. (2005) Annu. Rev. Immunol. 23:337.
  2. Steel, D. and A. Whitehead (1994) Immunol. Today 15:81.
  3. Alexandrescu, A.T. (2005) Protein Sci. 14:1.
  4. Emsley, J. et al. (1994) Nature 367:338.
  5. Mole, J.E. et al. (1988) J. Immunol. 141:3642.
  6. Gillmore, J.D. et al. (2004) Immunology 112:255.
  7. Pepys, M. et al. (2002) Nature 417:254.
  8. Bharadwaj, D. et al. (2001) J. Immunol. 166:6735.
  9. Szalai, A.J. et al. (2000) J. Immunol. 165:1030.
Long Name
Serum Amyloid P Component
Entrez Gene IDs
325 (Human); 20219 (Mouse); 29339 (Rat)
Alternate Names
9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; Pentraxin 2; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related; Serum Amyloid P component

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Citations for Mouse Pentraxin 2/SAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Regulatory T cells function in established systemic inflammation and reverse fatal autoimmunity
    Authors: W Hu, ZM Wang, Y Feng, M Schizas, BE Hoyos, J van der Ve, JG Verter, R Bou-Puerto, AY Rudensky
    Nature Immunology, 2021-08-23;22(9):1163-1174.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  2. Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus
    Authors: A Doni, R Parente, I Laface, E Magrini, C Cunha, FS Colombo, JF Lacerda, A Campos, SN Mapelli, F Petroni, R Porte, T Schorn, A Inforzato, T Mercier, K Lagrou, J Maertens, JD Lambris, B Bottazzi, C Garlanda, M Botto, A Carvalho, A Mantovani
    Nature Communications, 2021-06-18;12(1):3739.
    Species: Aspergillus, C. albicans
    Sample Types: Conidia, Hypheae
    Applications: Flow Cytometry

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