< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Mouse PTX2/SAP Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PTX2/SAP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse PTX2/SAP and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse PTX2/SAP showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse PTX2/SAP.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of PTX2/SAP spiked into cell culture samples was evaluated.
Average % Recovery
Cell Culture Samples (n=6)
To assess the linearity of the assay, samples containing high concentrations of mouse PTX2/SAP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Pentraxin 2/SAP
Pentraxin or Pentraxin 2 (PTX2), also known as Serum Amyloid P component (SAP), is a secreted serum glycoprotein belonging to the pentraxin superfamily. Pentraxins have a characteristic pentagonal discoid arrangement of five non-covalently bound subunits and bind a variety of molecules in a calcium- dependent lectin-like manner through a pattern-recognition-binding site. PTX2 is a major acute-phase protein and may function as an opsonin for a variety of ligands. PTX2 also associates ubiquitously with all amyloid deposits implicated in diseases including Alzheimer's and prion diseases, type 2 diabetes and various systemic amyloidoses.
Serum Amyloid P Component
Entrez Gene IDs
325 (Human); 20219 (Mouse); 29339 (Rat);
9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; Pentraxin 2; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related; Serum Amyloid P component;
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