Mouse Pentraxin 2/SAP Quantikine ELISA Kit

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MPTX20
Mouse Pentraxin 2/SAP ELISA Standard Curve
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Mouse Pentraxin 2/SAP Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.368 ng/mL
Assay Range
1.6 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse PTX2/SAP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse PTX2/SAP Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PTX2/SAP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse PTX2/SAP and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse PTX2/SAP showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse PTX2/SAP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.11 16 33.6 3.95 15.7 34.5
Standard Deviation 0.22 0.57 1.33 0.33 0.76 1.59
CV% 5.4 3.6 4 8.4 4.8 4.6

Recovery

The recovery of PTX2/SAP spiked into cell culture samples was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=6) 97 81-112

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse PTX2/SAP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse Pentraxin 2/SAP ELISA Linearity

Scientific Data

Mouse Pentraxin 2/SAP ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 2/SAP

Pentraxin or Pentraxin 2 (PTX2), also known as Serum Amyloid P component (SAP), is a secreted serum glycoprotein belonging to the pentraxin superfamily. Pentraxins have a characteristic pentagonal discoid arrangement of five non-covalently bound subunits and bind a variety of molecules in a calcium- dependent lectin-like manner through a pattern-recognition-binding site. PTX2 is a major acute-phase protein and may function as an opsonin for a variety of ligands. PTX2 also associates ubiquitously with all amyloid deposits implicated in diseases including Alzheimer's and prion diseases, type 2 diabetes and various systemic amyloidoses.

Long Name:
Serum Amyloid P Component
Entrez Gene IDs:
325 (Human); 20219 (Mouse); 29339 (Rat)
Alternate Names:
9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; Pentraxin 2; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related; Serum Amyloid P component
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Calibrator Diluent
  4.   Add 50 µL of Calibrator Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse Pentraxin 2/SAP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Increased susceptibility to organic dust exposure-induced inflammatory lung disease with enhanced rheumatoid arthritis-associated autoantigen expression in HLA-DR4 transgenic mice
    Authors: JA Poole, TR Mikuls, GM Thiele, R Gaurav, AJ Nelson, MJ Duryee, A Mitra, C Hunter, TA Wyatt, BR England, DP Ascherman
    Respiratory Research, 2022-06-18;23(1):160.
    Species: Mouse
    Sample Types: Serum
  2. The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung disease
    Authors: TR Mikuls, R Gaurav, GM Thiele, BR England, MG Wolfe, BP Shaw, KL Bailey, TA Wyatt, AJ Nelson, MJ Duryee, CD Hunter, D Wang, DJ Romberger, DP Ascherman, JA Poole
    International immunopharmacology, 2021-08-27;100(0):108069.
    Species: Mouse
    Sample Types: Serum
  3. LPS immune challenge reduces arcuate nucleus TSHR and CART mRNA and elevates plasma CART peptides
    Authors: JR Burgos, BM Iresjö, L Olsson, U Smedh
    BMC Neurosci, 2019-12-11;20(1):59.
    Species: Mouse
    Sample Types: Plasma
  4. Pathogenesis of Lethal Aspiration Pneumonia in Mecp2-null Mouse Model for Rett Syndrome
    Authors: H Kida, T Takahashi, Y Nakamura, T Kinoshita, M Hara, M Okamoto, S Okayama, K Nakamura, KI Kosai, T Taniwaki, Y Yamashita, T Matsuishi
    Sci Rep, 2017-09-20;7(1):12032.
    Species: Mouse
    Sample Types: Plasma

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