Mouse Podoplanin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF3244
Product Details
Citations (2)
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Mouse Podoplanin Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podoplanin in Western blots. In Western blots, approximately 1% cross-reactivity with recombinant human Podoplanin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
Gln21-Lys133
Accession # Q62011
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podoplanin

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Entrez Gene IDs
10630 (Human); 14726 (Mouse); 54320 (Rat)
Alternate Names
36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha

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Citations for Mouse Podoplanin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Single-cell RNA sequencing identifies response of renal lymphatic endothelial cells to acute kidney injury
    Authors: Creed, HA;Kannan, S;Tate, BL;Banerjee, P;Mitchell, BM;Chakraborty, S;Rutkowski, JM;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection
  2. Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells
    Authors: MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields
    Nat Commun, 2018-03-05;9(1):948.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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