Mouse Podoplanin Antibody

(13 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
    Gln21-Lys133
    Accession # Q546R8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Mouse Podoplanin by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Podoplanin in Mouse Kidney. Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Podoplanin

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

  • Long Name:
    Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
  • Entrez Gene IDs:
    10630 (Human); 14726 (Mouse); 54320 (Rat)
  • Alternate Names:
    36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro
    Authors: D Zheng, BS Soh, L Yin, G Hu, Q Chen, H Choi, J Han, VT Chow, J Chen
    Sci Rep, 2017;7(0):41661.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection
    Authors: CSE Tan, C Lawler, PG Stevenson
    PLoS Pathog., 2017;13(4):e1006311.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  3. Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1
    Authors: LA Johnson, S Banerji, W Lawrance, U Gileadi, G Prota, KA Holder, YM Roshorm, T Hanke, V Cerundolo, NW Gale, DG Jackson
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Type I Interferons Direct Gammaherpesvirus Host Colonization
    PLoS Pathog, 2016;12(5):e1005654.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  5. Aging exacerbates damage and delays repair of alveolar epithelia following influenza viral pneumonia.
    Authors: Yin L, Zheng D, Limmon G, Leung N, Xu S, Rajapakse J, Yu H, Chow V, Chen J
    Respir Res, 2015;15(0):116.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Effects of adenosine on lymphangiogenesis.
    Authors: Lenoir B, Wagner D, Blacher S, Sala-Newby G, Newby A, Noel A, Devaux Y
    PLoS ONE, 2014;9(3):e92715.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress.
    Authors: Sturrock, Anne, Mir-Kasimov, Mustafa, Baker, Jessica, Rowley, Jesse, Paine, Robert 3
    J Biol Chem, 2014;289(7):4095-105.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  8. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Regeneration of alveolar type I and II cells from Scgb1a1-expressing cells following severe pulmonary damage induced by bleomycin and influenza.
    Authors: Zheng, Dahai, Limmon, Gino V, Yin, Lu, Leung, Nicola H, Yu, Hanry, Chow, Vincent, Chen, Jianzhu
    PLoS ONE, 2012;7(10):e48451.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage.
    J. Immunol., 2012;189(5):2181-90.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation.
    Authors: Peters A, Pitcher LA, Sullivan JM, Mitsdoerffer M, Acton SE, Franz B, Wucherpfennig K, Turley S, Carroll MC, Sobel RA, Bettelli E, Kuchroo VK
    Immunity, 2011;35(6):986-96.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. The Appearance and Modulation of Osteocyte Marker Expression during Calcification of Vascular Smooth Muscle Cells.
    Authors: Zhu D, Mackenzie NC, Millan JL, Farquharson C, MacRae VE
    PLoS ONE, 2011;6(5):e19595.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3.
    Authors: Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivee B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A
    J. Cell Biol., 2010;188(1):115-30.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
Expand to show all 13 Citations
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