Mouse Pygopus-1 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Pygopus-1

Pygopus (the name of a legless Australian lizard) is a 45 kDa transcription co-activator that functions in the Wnt signaling pathway. Mouse Pygopus-1 is a nuclear protein that is synthesized as a 417 amino acid residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) finger Zn²⁺-binding domain. A nuclear localization signal is present within the NHD. The PHD finger of Pygopus binds to Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formation of the quaternary complex is required for Wnt signaling. Mouse and human Pygopus-1 share 87% amino acid sequence homology.

Product Datasheets

Related Research Areas

• Stem Cell Transcription Factor Regulators
• Transcription Factors and Regulators
• Transcriptional Co-Activators
• Wnt Family
• Wnt Intracellular Signaling