Mouse Pygopus-1 Antibody

R&D Systems | Catalog # AF3533

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pygopus-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Pygopus-1
Ser2-Ala417
Accession # Q9D0P5

Specificity

Detects mouse Pygopus-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Pygopus-1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Pygopus‑1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pygopus-1

Pygopus (the name of a legless Australian lizard) is a 45 kDa transcription co-activator that functions in the Wnt signaling pathway. Mouse Pygopus-1 is a nuclear protein that is synthesized as a 417 amino acid residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) finger Zn2+-binding domain. A nuclear localization signal is present within the NHD. The PHD finger of Pygopus binds to Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formation of the quaternary complex is required for Wnt signaling. Mouse and human Pygopus-1 share 87% amino acid sequence homology.

Alternate Names

Pygo1, Pygopus1

Entrez Gene IDs

26108 (Human); 72135 (Mouse)

Gene Symbol

PYGO1

UniProt

Q9D0P5

Additional Pygopus-1 Products

Product Documents for Mouse Pygopus-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Pygopus-1 Antibody

For research use only

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