Mouse R-Spondin 3 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB4120G Datasheet / COA / SDS

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FAB4120G-100UG

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Mouse R-Spondin 3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse R‑Spondin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) RSPO-1, rmRSPO-4, recombinant human (rh) RSPO-2, or rhRSPO-3 is observed.

Source

Monoclonal Rat IgG2a Clone # 400404

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse Râ€‘Spondin 3
Ser21-Gly209 (Gln108Lys)
Accession # BAC36296

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: R-Spondin 3

RSPO-3 (R-spondin 3; also Cristin-1), is a 256 aa secreted R-Spondin family member which binds LRP-6 and Fzd8 and regulates beta -catenin signaling. R-Spondins contain two adjacent cysteine-rich furin-like domains at their amino-termini, followed by a thrombospondin (TSP-1) motif and a variable length basic C-terminal region. Two RSPO-3 splice isoforms that differ in their C-termini have been reported. Mouse RSPO-3 shares 93% and 97% aa identity with human and rat RSPO-3, respectively, over the sequence used as an immunogen.

Long Name

Roof Plate-specific Spondin 3

Entrez Gene IDs

84870 (Human); 72780 (Mouse); 102132009 (Cynomolgus Monkey)

Alternate Names

Cristin 1; CRISTIN1; FLJ14440; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; Roof plate-specific spondin-3; RSPO3; R-spondin 3 homolog (Xenopus laevis); RSpondin 3; R-Spondin 3; R-spondin-3; Thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2; THSD2