Mouse Rae-1 alpha / beta / gamma Antibody

Catalog # Availability Size / Price Qty
MAB1758
MAB1758-SP

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Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (4)
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Mouse Rae-1 alpha / beta / gamma Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1 alpha / beta / gamma. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody recognizes mouse Rae-1 alpha, beta and gamma. It does not recognize Rae-1δ or epsilon.
Source
Monoclonal Rat IgG2A Clone # 199215
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with Rae-1 beta and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Mouse NKG2D Fc Chimera (Catalog # 139-NK) to immobilized Recombinant Mouse Rae-1 beta Fc Chimera (Catalog # 1198-RA) coated at 1 µg/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding. This antibody also blocks the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha and Rae-1 gamma.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Rae-1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 alpha/beta/gamma Monoclonal Antibody (Catalog # MAB1758, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rae-1

The Rae-1 family is a group of closely related GPI-linked proteins that function as ligands for NKG2D. Binding of Rae-1 proteins to NKG2D results in the activation of cytolytic activity and/or cytokine production by effector T cells and NK cells.

Long Name
Retinoic Acid Early Transcript 1
Entrez Gene IDs
8480 (Human); 19368 (Mouse)
Alternate Names
Rae1; Rae-1

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Citations for Mouse Rae-1 alpha / beta / gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Molecular Signatures of Dengue Virus-Specific IL-10/IFN-gamma Co-producing CD4�T Cells and Their Association with Dengue Disease
    Authors: Y Tian, G Seumois, LM De-Oliveir, J Mateus, S Herrera-de, C Kim, D Hinz, NDS Goonawardh, AD de Silva, S Premawansa, G Premawansa, A Wijewickra, A Balmaseda, A Grifoni, P Vijayanand, E Harris, B Peters, A Sette, D Weiskopf
    Cell Rep, 2019;29(13):4482-4495.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity.
    Authors: Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N
    Leukemia, 2014;28(6):1316-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Ras activation induces expression of Raet1 family NK receptor ligands.
    J. Immunol., 2012;189(4):1826-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Tripeptidyl-peptidase II controls DNA damage responses and in vivo gamma-irradiation resistance of tumors.
    Authors: Hong X, Lei L, Kunert B, Naredla R, Applequist SE, Grandien A, Glas R
    Cancer Res., 2007;67(15):7165-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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