Mouse/Rat DDT Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse and Rat DDT by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Mouse/Rat DDT Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat DDT in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse DDT
Accession # O35215
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat DDT antibody by Western Blot. View Larger

Detection of Mouse and Rat DDT by Western Blot. Western blot shows lysates of mouse liver tissue, mouse testis tissue, mouse kidney tissue, rat liver tissue, rat testis tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat DDT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8354) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DDT at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDT

Mouse DDT (D-dopachrome tautomerase; also D-dopachrome decarboxylase and Phenylpyruvate tautomerase) is a 13 kDa member of the carboxy-lyases family. The DDT gene in mouse, rat and human is identical in exon structure to the Macrophage migration inhibitory factor (MIF) gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure. The genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10. DDT mRNA levels in human adipocytes are negatively correlated with obesity. The study on DDT knock-down adipocytes shows an increase in the expression of genes involved in both lipolysis and lipogenesis. DDT binds CD74 with high affinity, leading to activation of ERK1/2 MAP kinase and downstream pro-inflammatory pathways. Circulating levels of DDT correlate with disease severity in sepsis or malignancy. DDT also inhibits chemotaxis induced by CCL2 in macrophages. DDT in tumor angiogenesis promotes the expression of pro-angiogenic factors CXCL8 (IL8) and VEGF-A in non-small cell lung carcinoma cells through the CD74 receptor.  Over aa 2-118, mouse DDT shares 95.7% and 73.5% aa identity with rat and human DDT, respectively.  

Long Name
D-Dopachrome Tautomerase
Entrez Gene IDs
1652 (Human); 13202 (Mouse); 29318 (Rat)
Alternate Names
DDCT; D-Dopachrome Decarboxylase; D-dopachrome tautomeraseDDCT; DDT; DOPD; EC; MIF2; Phenylpyruvate Tautomerase II

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Citation for Mouse/Rat DDT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MIF homolog d-dopachrome tautomerase (D-DT/MIF-2) does not inhibit accumulation and toxicity of misfolded SOD1
    Authors: A Alaskarov, S Barel, S Bakavayev, J Kahn, A Israelson
    Oncogene, 2022-06-10;12(1):9570.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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