Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit

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RFBP10
Mouse/Rat FABP1/L-FABP ELISA Standard Curve
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Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Sensitivity
0.029 ng/mL
Assay Range
0.1 - 5 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant FABP1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat FABP1 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FABP1 in mouse or rat cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant rat FABP1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant rat FABP1. Results obtained using natural mouse or rat FABP1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural FABP1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.28 0.69 1.22 0.27 0.68 1.25
Standard Deviation 0.01 0.02 0.03 0.01 0.03 0.06
CV% 3.6 2.9 2.5 3.7 4.4 4.8

Recovery

The recovery of FABP1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 90-100
Mouse Urine (n=4) 96 86-108
Rat EDTA Plasma (n=4) 94 84-107
Rat Heparin Plasma (n=4) 100 92-111
Rat Serum (n=4) 100 87-116
Rat Urine (n=4) 99 95-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FABP1 in each matrix were diluted with Calibrator Diluent and assayed.
Mouse/Rat FABP1/L-FABP ELISA Linearity
Mouse/Rat FABP1/L-FABP ELISA Linearity

Data Examples

Mouse/Rat FABP1/L-FABP ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP1/L-FABP

Fatty Acid Binding Protein 1, also known as liver FABP, is a cytoplasmic transporter for long chain fatty acids, oxidized/peroxidized fatty acids, cholesterol, heme, Vitamin K, steroids, acyl CoA, leukotrienes, and prostaglandins. It is expressed by hepatocytes, simple columnar epithelium of the small intestine, and renal proximal tubule epithelium. FABP1 trafficks between the cytosol, peroxisomes, and nucleus. It delivers fatty acid cargo to the transcription factor PPAR alpha, leading to increased production of enzymes involved in the beta-oxidation of fatty acids. FABP1 also plays a role in the formation of prechylomicron vesicles by the endoplasmic reticulum.

Long Name:
Fatty Acid-Binding Protein 1
Entrez Gene IDs:
2168 (Human); 14080 (Mouse); 24360 (Rat)
Alternate Names:
FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury
    Authors: GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov
    PLoS ONE, 2017;12(2):e0171703.
    Species: Mouse
    Sample Types: Plasma
  2. Sex differences in the excretion levels of traditional and novel urinary biomarkers of nephrotoxicity in rats
    Authors: S Tsuji, M Sugiura, S Tsutsumi, H Yamada
    J Toxicol Sci, 2017;42(5):615-627.
    Species: Rat
    Sample Types: Urine
  3. An Atherogenic Paigen-Diet Aggravates Nephropathy in Type 2 Diabetic OLETF Rats.
    Authors: Nozako M, Koyama T, Nagano C, Sato M, Matsumoto S, Mitani K, Yasufuku R, Kohashi M, Yoshikawa T
    PLoS ONE, 2015;10(11):e0143979.

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