Mouse/Rat IL-17F Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
M17F0
Mouse/Rat IL-17F Standard Curve
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Mouse/Rat IL-17F Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
1.74 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat IL-17F.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat IL-17F Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-17F in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-17F and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-17F showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-17F.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 56.7 211 409 53.2 202 399
Standard Deviation 1.26 4.93 13.1 3.98 11.4 20.2
CV% 2.2 2.3 3.2 7.5 5.6 5.1

Recovery

The recovery of mouse IL-17F spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 92-99
EDTA Plasma (n=4) 107 101-118
Heparin Plasma (n=4) 101 88-121
Serum (n=4) 95 83-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17F in each matrix were diluted with Calibrator Diluent and assayed.
Mouse/Rat IL-17F ELISA Linearity

Data Examples

Mouse/Rat IL-17F Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17F

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:
Interleukin 17F
Entrez Gene IDs:
112744 (Human); 257630 (Mouse); 301291 (Rat)
Alternate Names:
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citation for Mouse/Rat IL-17F Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interleukin-17A is involved in mechanical hyperalgesia but not in the severity of murine antigen-induced arthritis
    Authors: M Ebbinghaus, G Natura, G Segond von, S Hensellek, M Böttcher, B Hoffmann, FS Salah, M Gajda, T Kamradt, HG Schaible
    Sci Rep, 2017;7(1):10334.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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