Mouse/Rat Nestin PE-conjugated Antibody

(5 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat Nestin in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 307501
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant rat Nestin
    Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
    Accession # EDM00749
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Nestin in Mouse Cortical Stem Cells by Flow Cytometry. Mouse cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of Nestin in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Nestin
Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14). Over amino acids (aa) 544-820, rat Nestin shares 75% and 38% sequence identity with mouse and human Nestin, respectively.
  • References:
    1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
    2. Lendahl, U. et al. (1990) Cell 60:585.
    3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
    4. Tohyama, T. et al. (1992) Lab. Invest. 66:303.
    5. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
    6. Frederiksen, K. et al. (1988) Neuron 1:439.
    7. Cattaneo, E. et al. (1990) Nature 347:762.
    8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
    9. Rietze, R.L. et al. (2001) Nature 412:736.
    10. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
    11. Zulewski, H. et al. (2001) Diabetes 50:521.
    12. Lumelsky, N. et al. (2001) Science 292:1389.
    13. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
    14. Shih, C.C. et al. (2001) Blood 98:2412.
  • Entrez Gene IDs:
    10763 (Human); 18008 (Mouse); 25491 (Rat)
  • Alternate Names:
    FLJ21841; NES; Nestin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals
    Authors: Y Wang, X Ying, H Xu, H Yan, X Li, H Tang
    Int J Nanomedicine, 2017;12(0):1369-1384.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile
    J Immunol, 2016;196(11):4760-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
    Authors: Hu S, Rotschafer J, Lokensgard J, Cheeran M
    PLoS ONE, 2014;9(8):e105219.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
    Authors: Mutnal MB, Cheeran MC, Hu S
    PLoS ONE, 2011;6(1):e16211.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Staining Reagents
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Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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