Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 307501
View all Nestin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749

Specificity

Detects mouse and rat Nestin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Mouse/Rat Nestin Antibody

Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

Nestin in Rat Cortical Stem Cells.

Nestin was detected in immersion fixed undifferentiated rat cortical stem cells using Mouse Anti-Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse Nestin by Immunocytochemistry/Immunofluorescence

Detection of Mouse Nestin by Immunocytochemistry/Immunofluorescence

Zfp322a can enhance OSKM reprogramming and replace Sox2.(A) Zfp322a enhanced reprogramming efficiency and accelerated the onset of reprogramming process. OSKM serves as control experiment. (B) The iPSCs generated from OSKM plus Zfp322a presented alkaline phosphatase activity. There were more AP stained colonies generated from OKSM+Zfp322a compare to OKSM. (C) The iPSCs expressed endogenous Oct4, Nanog, Sox2, Rex1 and SSEA-1, indicating that they were ES-cell like. Immunostaining using anti-Oct4, anti-Nanog anti-Sox2, anti-Rex1 and anti-SSEA-1 antibodies were performed with GFP+ iPSCs generated from OKSM+Zfp322a. (D) GFP+ iPSCs generated by OKSM+Zfp322a were able to express ectoderm, mesoderm and endoderm lineage markers in the EB formation assay. iPSCs were stained with anti-Nestin, anti-Gata4 and anti-alpha smooth muscle actin (SMA) antibodies and pictures were taken at 60× magnification. DAPI (blue) served as nucleus marker. (E) Zfp322a was able to replace Sox2, but not Oct4 or Klf4 in OSKM reprogramming process. Results from three independent experiments were presented. (F) iPSCs generated from OKM plus Zfp322a were positive with AP staining and more AP positive colonies were observed in OKM+Zfp322a as compared to OKSM. (G) iPSCs generated by OKM plus Zfp322a expressed pluripotency markers Oct4, Nanog, Sox2, Rex1 and SSEA-1. (H) iPSCs derived from OKM+Zfp322a could differentiate into ectoderm, mesoderm and endoderm lineages, which were showed by anti-Nestin, anti-Gata4, anti-SMA staining respectively. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1004038), licensed under a CC-BY license. Not internally tested by R&D Systems.

Nestin in C2C12 Mouse Cell Line.

Nestin was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Nestin by Immunocytochemistry/ Immunofluorescence

Detection of Nestin by Immunocytochemistry/ Immunofluorescence

Zfp322a can enhance OSKM reprogramming and replace Sox2.(A) Zfp322a enhanced reprogramming efficiency and accelerated the onset of reprogramming process. OSKM serves as control experiment. (B) The iPSCs generated from OSKM plus Zfp322a presented alkaline phosphatase activity. There were more AP stained colonies generated from OKSM+Zfp322a compare to OKSM. (C) The iPSCs expressed endogenous Oct4, Nanog, Sox2, Rex1 and SSEA-1, indicating that they were ES-cell like. Immunostaining using anti-Oct4, anti-Nanog anti-Sox2, anti-Rex1 and anti-SSEA-1 antibodies were performed with GFP+ iPSCs generated from OKSM+Zfp322a. (D) GFP+ iPSCs generated by OKSM+Zfp322a were able to express ectoderm, mesoderm and endoderm lineage markers in the EB formation assay. iPSCs were stained with anti-Nestin, anti-Gata4 and anti-alpha smooth muscle actin (SMA) antibodies and pictures were taken at 60× magnification. DAPI (blue) served as nucleus marker. (E) Zfp322a was able to replace Sox2, but not Oct4 or Klf4 in OSKM reprogramming process. Results from three independent experiments were presented. (F) iPSCs generated from OKM plus Zfp322a were positive with AP staining and more AP positive colonies were observed in OKM+Zfp322a as compared to OKSM. (G) iPSCs generated by OKM plus Zfp322a expressed pluripotency markers Oct4, Nanog, Sox2, Rex1 and SSEA-1. (H) iPSCs derived from OKM+Zfp322a could differentiate into ectoderm, mesoderm and endoderm lineages, which were showed by anti-Nestin, anti-Gata4, anti-SMA staining respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24550733), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse/Rat Nestin Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed undifferentiated rat cortical stem cells and C2C12 mouse myoblast cell line

Western Blot

1 µg/mL
Sample: Recombinant Rat Nestin

Reviewed Applications

Read 4 reviews rated 4.8 using MAB2736 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nestin

Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

References

  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.

Alternate Names

NES

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Gene Symbol

NES

UniProt

EDM00749

Additional Nestin Products

Product Documents for Mouse/Rat Nestin Antibody

Certificate of Analysis

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Product Specific Notices for Mouse/Rat Nestin Antibody

For research use only

Citations for Mouse/Rat Nestin Antibody

Customer Reviews for Mouse/Rat Nestin Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • Mouse/Rat Nestin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS cells
    Species: Mouse
    Verified Customer | Posted 10/26/2021
    Mouse/Rat Nestin Antibody MAB2736
  • Mouse/Rat Nestin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: epithelial cells
    Species: Mouse
    Verified Customer | Posted 09/04/2021
    Mouse/Rat Nestin Antibody MAB2736
  • Mouse/Rat Nestin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neural stem cells derived from h9
    Species: Human
    Verified Customer | Posted 08/19/2019
    Neural stem cells were fixed in 4%PFA for 15 minutes. The nestin antibody was used at 10ug/ml at 4 degree overnight. Secondary antibody was incubated at room temperature for 1 hour.
    Mouse/Rat Nestin Antibody MAB2736
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 23798370
    Species: Rat
    Verified Customer | Posted 02/19/2015

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