Mouse/Rat Nestin Antibody

(8 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat Nestin in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 307501
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant rat Nestin
    Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
    Accession # EDM00749
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Rat Nestin
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed undifferentiated rat cortical stem cells using Mouse Anti‑Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nestin
Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
  • References:
    1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
    2. Lendahl, U. et al. (1990) Cell 60:585.
    3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
    4. Tohyama, T. et al. (1992) Lab. Invest. 66:303.
    5. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
    6. Frederiksen, K. et al. (1988) Neuron 1:439.
    7. Cattaneo, E. et al. (1990) Nature 347:762.
    8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
    9. Rietze, R.L. et al. (2001) Nature 412:736.
    10. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
    11. Zulewski, H. et al. (2001) Diabetes 50:521.
    12. Lumelsky, N. et al. (2001) Science 292:1389.
    13. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
    14. Shih, C.C. et al. (2001) Blood 98:2412.
  • Entrez Gene IDs:
    10763 (Human); 18008 (Mouse); 25491 (Rat)
  • Alternate Names:
    FLJ21841; NES; Nestin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy
    Authors: SB Ross, ST Fraser, RD Bagnall, C Semsarian
    Stem Cell Res, 2017;20(0):76-79.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. The PD-1/B7-H1 pathway modulates the natural killer cells versus mouse glioma stem cells.
    Authors: Huang B, Zhan Y, Zong W, Yu C, Li J, Qu Y, Han S
    PLoS ONE, 2015;10(8):e0134715.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  4. The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation.
    Authors: Lyu J, Yu X, He L, Cheng T, Zhou J, Cheng C, Chen Z, Cheng G, Qiu Z, Zhou W
    Mol Brain, 2015;8(0):26.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  5. Nestin delineates pancreatic cancer stem cells in metastatic foci of NOD/Shi-scid IL2Rgamma(null) (NOG) mice.
    Authors: Matsuda Y, Yoshimura H, Ueda J, Naito Z, Korc M, Ishiwata T
    Am J Pathol, 2014;184(3):674-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm.
    Authors: Engels M, Rajarajan K, Feistritzer R, Sharma A, Nielsen U, Schalij M, de Vries A, Pijnappels D, Wu S
    Stem Cells, 2014;32(6):1493-502.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. The pleiotrophin-ALK axis is required for tumorigenicity of glioblastoma stem cells.
    Authors: Koyama-Nasu, R, Haruta, R, Nasu-Nishimura, Y, Taniue, K, Katou, Y, Shirahige, K, Todo, T, Ino, Y, Mukasa, A, Saito, N, Matsui, M, Takahashi, R, Hoshino-Okubo, A, Sugano, H, Manabe, E, Funato, K, Akiyama, T
    Oncogene, 2014;33(17):2236-44.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. VEGF-mediated angiogenesis stimulates neural stem cell proliferation and differentiation in the premature brain.
    Authors: Sun J, Sha B, Zhou W, Yang Y
    Biochem. Biophys. Res. Commun., 2010;394(1):146-52.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
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