Mouse/Rat Neurocan Antibody

Catalog # Availability Size / Price Qty
AF5800
AF5800-SP
Detection of Mouse/Rat Neurocan by Western Blot.
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Product Details
Citations (12)
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Mouse/Rat Neurocan Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Neurocan in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Neurocan
Asp23-Asp637
Accession # NP_031815
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat Neurocan antibody by Western Blot. View Larger

Detection of Mouse/Rat Neurocan by Western Blot. Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurocan at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Neurocan antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

Neurocan in Mouse Brain. Neurocan was detected in perfusion fixed frozen sections of mouse brain (medulla) using Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurocan

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37-157), two Link domains (aa 159-254 and 258-356), two EGF-like domains (aa 960-996 and 998-1034), one C-type lectin-like domain (aa 1036-1165), one Sushi domain (aa 1165-1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2-3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).

References
  1. Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350.
  2. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
  3. Retzler, C. et al. (1996) J. Biol. Chem. 271:27304.
  4. Quaglia, X. et al. (2008) Brain 131:240.
Entrez Gene IDs
1463 (Human); 13004 (Mouse); 58982 (Rat)
Alternate Names
chondroitin sulfate proteoglycan 3 (neurocan); Chondroitin sulfate proteoglycan 3; CSPG3; CSPG3neurocan core protein; FLJ44681; NCAN; NEUR; neurocan proteoglycan; Neurocan

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Citations for Mouse/Rat Neurocan Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Astrocyte-Secreted Neurocan Controls Inhibitory Synapse Formation and Function
    Authors: D Irala, S Wang, K Sakers, L Nagendren, FP Ulloa-Seve, DS Bindu, C Eroglu
    bioRxiv : the preprint server for biology, 2023-04-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  2. Distribution and postnatal development of chondroitin sulfate proteoglycans in the perineuronal nets of cholinergic motoneurons innervating extraocular muscles
    Authors: A Ritok, P Kiss, A Zaher, E Wolf, L Ducza, T Bacskai, C Matesz, B Gaal
    Scientific Reports, 2022-12-14;12(1):21606.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Brevican and Neurocan Cleavage Products in the Cerebrospinal Fluid - Differential Occurrence in ALS, Epilepsy and Small Vessel Disease
    Authors: W Hu beta ler, L Höhn, C Stolz, S Vielhaber, C Garz, FC Schmitt, ED Gundelfing, S Schreiber, CI Seidenbech
    Frontiers in Cellular Neuroscience, 2022-04-11;16(0):838432.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  4. Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies
    Authors: T Fontanil, Y Mohamedi, A Moncada-Pa, T Cobo, JA Vega, JL Cobo, O García-Suá, J Cobo, ÁJ Obaya, S Cal
    Cell. Physiol. Biochem., 2019-01-01;52(5):1003-1016.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  5. Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices
    Authors: H Ueno, S Suemitsu, S Murakami, N Kitamura, K Wani, Y Matsumoto, M Okamoto, T Ishihara
    IBRO Rep, 2018-11-28;6(0):1-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets
    Authors: M Sonntag, M Blosa, S Schmidt, K Reimann, K Blum, T Eckrich, G Seeger, D Hecker, B Schick, T Arendt, J Engel, M Morawski
    BMC Biol., 2018-09-26;16(1):99.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  7. BMP-responsive Protease HtrA1 is Differentially Expressed in Astrocytes and Regulates Astrocytic Development and Injury Response
    Authors: J Chen, S Van Gulden, TL McGuire, AC Fleming, C Oka, JA Kessler, CY Peng
    J. Neurosci., 2018-02-26;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex
    Authors: H Ueno, S Suemitsu, M Okamoto, Y Matsumoto, T Ishihara
    Neuroscience, 2017-05-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates
    PLoS ONE, 2016-11-03;11(11):e0166119.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem Nuclei.
    Authors: Ebbers L, Satheesh S, Janz K, Ruttiger L, Blosa M, Hofmann F, Morawski M, Griesemer D, Knipper M, Friauf E, Nothwang H
    J Biol Chem, 2015-08-04;290(39):23692-710.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Neurons and glia modify receptor protein-tyrosine phosphatase zeta (RPTPzeta)/phosphacan with cell-specific O-mannosyl glycans in the developing brain.
    Authors: Dwyer C, Katoh T, Tiemeyer M, Matthews R
    J Biol Chem, 2015-03-03;290(16):10256-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
    Authors: Bekku Y, Oohashi T
    Arch. Histol. Cytol., 2010-01-01;73(2):95-102.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot

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