Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant human Neuropilin‑1 is observed, and less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Phe22-Ala810 (Lys811Arg), Ser829-Asp854 Accession # Q9QWJ9
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse/Rat Neuropilin‑1 Alexa Fluor® 700‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566N, filled histogram) or isotype control antibody (Catalog # IC108N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Neuropilin‑1 in PC‑12 Rat Cell Line by Flow Cytometry.
PC‑12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Mouse/Rat Neuropilin‑1 Alexa Fluor® 700‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566N, filled histogram) or isotype control antibody (Catalog # IC108N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.
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