Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse, Rat, Avian - Chicken, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cell Culture, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Neuropilin-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
Phe22-Ala810 (Lys811Arg), Ser829-Asp854
Accession # Q9QWJ9

Specificity

Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse/Rat Neuropilin‑1 Antibody

Detection of Recombinant Human, Mouse, and Rat Neuropilin-1 antibody by Western Blot.

Detection of Recombinant Human, Mouse, and Rat Neuropilin‑1 by Western Blot.

Western blot shows 25 ng of Recombinant Mouse Neuropilin-1 (Catalog # 5994-N1), Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin-1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Neuropilin-1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Neuropilin-1 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Neuropilin-1 antibody in Embryonic Rat Spinal Cord by Immunohistochemistry (IHC-Fr).

Neuropilin‑1 in Embryonic Rat Spinal Cord.

Neuropilin-1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neuropilin-1 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr).

Neuropilin‑1 in Rat Spinal Cord.

Neuropilin-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Neuropilin-1 antibody in Rat Brain by Immunohistochemistry (IHC-P).

Neuropilin‑1 in Rat Brain.

Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of rat brain (hippocampus) using Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Neuropilin-1 antibody in Rat Brain by Immunohistochemistry (IHC-P).

Neuropilin‑1 in Rat Brain.

Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse Neuropilin-1 by Western Blot

Detection of Mouse Neuropilin-1 by Western Blot

Nrp1 influences Smad2/3 activation.(a–c) Representative confocal images of wt sprouts from EBs immunolabelled for pSmad2. The outline of the wt sprouts is indicated with a dashed line using the endogenous DsRED marker. DAPI staining (not shown) was used to mark nuclei (blue line). Sprouts from (a) untreated EBs, (b) treated with 2 ng ml−1 Tgf-beta for 1 h and(c) treated with 10 μM SB-421543 for 4 h. Scale bar, 13 μm. (d,e) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Fig. 10. A representative blot of six is shown; P=0.0012 NRP1 siRNA compared with control. (f,g) Proteins from P4 HUVEC transfected with control-GFP and NRP1–GFP–His construct for 24 h, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h were assessed for SMAD2 phosphorylation. Full western blots are shown in Supplementary Fig. 11. A representative blot of four is shown; P=0.0017 NRP1 overexpression compared with control. (e,g) Quantification of pSMAD2 protein normalized to SMAD2/3. (h–k) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Figs 12 and 13. (i) Quantification of pSMAD2 protein normalized to SMAD2/3; P=0.0848 Nrp1 siRNA compared with control. (k) Quantification of pSMAD3 protein normalized to SMAD3; P=0.0181 NRP1 siRNA compared with control. (l,m) Western blot analysis of P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 10 ng ml−1 BMP9 for 15 and 30 min. Full western blots are shown in Supplementary Fig. 14. A representative blot of four is shown. (m) Quantification of pSMAD2/3 protein normalized to SMAD2; P<0.0011 NRP1 siRNA compared with control. All values represent mean±s.e.m. DAPI, 4,6-diamidino-2-phenylindole; NS, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26081042), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Neuropilin-1 by Western Blot

Detection of Mouse Neuropilin-1 by Western Blot

Expression of candidate genes. (A) qPCR analysis of relative mRNA levels in total RNA from isolated glomeruli of Foxc2fl/fl; Pod‐Cre+, Foxc2fl/+; Pod‐Cre+ and Foxc2fl/+ mice. Expression levels were normalized against the expression of Rplp0. (B) qPCR analysis of relative mRNA levels in total RNA from a podocyte cell line treated with either Foxc2 siRNA or non‐targeting siRNA. (C) Western blot analysis of NRP1 and ITGB1 protein expression after Foxc2 induction (pBABE‐Foxc2) or knockdown (Foxc2 siRNA). Empty vector (pBABE) and non‐targeting siRNA was used as controls. *P < 0.05 Foxc2fl/fl;Pod‐Cre+ versus Foxc2fl/+. Error bars in (A) and (B) represent SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31062503), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Neuropilin-1 by Western Blot

Detection of Mouse Neuropilin-1 by Western Blot

Nrp1 influences Smad2/3 activation.(a–c) Representative confocal images of wt sprouts from EBs immunolabelled for pSmad2. The outline of the wt sprouts is indicated with a dashed line using the endogenous DsRED marker. DAPI staining (not shown) was used to mark nuclei (blue line). Sprouts from (a) untreated EBs, (b) treated with 2 ng ml−1 Tgf-beta for 1 h and(c) treated with 10 μM SB-421543 for 4 h. Scale bar, 13 μm. (d,e) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Fig. 10. A representative blot of six is shown; P=0.0012 NRP1 siRNA compared with control. (f,g) Proteins from P4 HUVEC transfected with control-GFP and NRP1–GFP–His construct for 24 h, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h were assessed for SMAD2 phosphorylation. Full western blots are shown in Supplementary Fig. 11. A representative blot of four is shown; P=0.0017 NRP1 overexpression compared with control. (e,g) Quantification of pSMAD2 protein normalized to SMAD2/3. (h–k) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Figs 12 and 13. (i) Quantification of pSMAD2 protein normalized to SMAD2/3; P=0.0848 Nrp1 siRNA compared with control. (k) Quantification of pSMAD3 protein normalized to SMAD3; P=0.0181 NRP1 siRNA compared with control. (l,m) Western blot analysis of P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 10 ng ml−1 BMP9 for 15 and 30 min. Full western blots are shown in Supplementary Fig. 14. A representative blot of four is shown. (m) Quantification of pSMAD2/3 protein normalized to SMAD2; P<0.0011 NRP1 siRNA compared with control. All values represent mean±s.e.m. DAPI, 4,6-diamidino-2-phenylindole; NS, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26081042), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Neuropilin-1 by Western Blot

Detection of Neuropilin-1 by Western Blot

Rescue of VEGF-A Signaling in Nrp1cyto and Nrp1fl/fl Primary EC by Full-Length NRP1 and of EC Tubulogenesis following NRP1 siRNA Knockdown with Constitutively Active ERK(A and B) Western blot analysis of Nrp1cyto EC that were transduced with the indicated adenoviral constructs, serum-starved and stimulated with 50 ng/ml VEGF-A165. Blots (A) and quantitation of ERK activation (B) show that Ad-Nrp1, but not Ad-Nrp1cyto or Ad-Nrp1PDZ, restores VEGF-A-induced VEGFR2 and ERK activation in Nrp1cyto EC (mean ± SD, n = 3, *p < 0.05).(C and D) Western blot analysis of Nrp1fl/fl EC treated with adenoviral constructs expressing CRE recombinase to inactivate NRP1 were transduced with the indicated adenoviral constructs, serum-starved and stimulated with 50 ng/ml VEGF-A165. Blots (C) and quantitation (D) show that Ad-Nrp1, but not Ad-Nrp1cyto or Ad-Nrp1PDZ, restores the activation of VEGFR2 and ERK (mean ± SD, n = 3, *p < 0.05).(E and F) HUVEC treated with control or NRP1 siRNA were treated with the indicated adenoviral vectors to compare their capacity to undergo tube formation in 3D collagen matrices after 72 hr. Representative images (E) and quantification (F) of tubulogenesis after 72 hr (mean ± SEM, n = 18, *p < 0.05). Scale bar represents 100 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Neuropilin-1 by Western Blot

Detection of Neuropilin-1 by Western Blot

Impaired VEGF-A Signaling in Nrp1cyto Mice(A) Western blot analysis of heart lysates following intraperitoneal injection of VEGF-A. Blots show reduced ERK and VEGFR2 phosphorylation in Nrp1cyto mice relative to WT littermates.(B–D) Western blot analysis of cell lysates from primary arterial EC from Nrp1cyto and WT EC that were serum-starved and then stimulated for the indicated times with 50 ng/ml VEGF-A165. (B) Blots show reduced phosphorylation of VEGFR2 on Y1175 and of ERK in Nrp1cyto relative to control EC. (C) Quantification of the ratio of phospho-VEGFR2 (pVEGFR2) relative to total VEGFR2 (VEGFR2) (mean ± SD, n = 5, *p < 0.05). (D) Quantification of the ratio of phospho-ERK (pERK) relative to total ERK (ERK) (mean ± SD, n = 5, *p < 0.05).(E) Western blot analysis of cell lysates from Nrp1cyto and WT primary arterial EC that were serum-starved and stimulated for the indicated times with 50 ng/ml of FGF2 or IGF1. ERK phosphorylation was similar in Nrp1cyto and WT EC.See also Figure S4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Neuropilin-1 by Western Blot

Detection of Neuropilin-1 by Western Blot

Impaired VEGF-A Signaling in Nrp1cyto Mice(A) Western blot analysis of heart lysates following intraperitoneal injection of VEGF-A. Blots show reduced ERK and VEGFR2 phosphorylation in Nrp1cyto mice relative to WT littermates.(B–D) Western blot analysis of cell lysates from primary arterial EC from Nrp1cyto and WT EC that were serum-starved and then stimulated for the indicated times with 50 ng/ml VEGF-A165. (B) Blots show reduced phosphorylation of VEGFR2 on Y1175 and of ERK in Nrp1cyto relative to control EC. (C) Quantification of the ratio of phospho-VEGFR2 (pVEGFR2) relative to total VEGFR2 (VEGFR2) (mean ± SD, n = 5, *p < 0.05). (D) Quantification of the ratio of phospho-ERK (pERK) relative to total ERK (ERK) (mean ± SD, n = 5, *p < 0.05).(E) Western blot analysis of cell lysates from Nrp1cyto and WT primary arterial EC that were serum-starved and stimulated for the indicated times with 50 ng/ml of FGF2 or IGF1. ERK phosphorylation was similar in Nrp1cyto and WT EC.See also Figure S4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Neuropilin-1 by Western Blot

Detection of Neuropilin-1 by Western Blot

Rescue of Defective ERK Signaling in VEGF-A-Stimulated Nrp1cyto Arterial EC by Knockdown of PTP1bPrimary arterial EC from Nrp1cyto mice transfected with siRNA specific for the indicated phosphatases were serum-starved and then stimulated with 50 ng/ml VEGF-A165.(A and B) Knockdown of the indicated phosphatases in Nrp1cyto arterial EC shown by immunoblotting (A); PTP1b knockdown was quantified in (B), dashed line indicates normal expression levels.(C and D) ERK and VEGFR2 (Y1175) phosphorylation after knockdown of the indicated phosphatases shown by immunoblotting (C). Quantification of pERK activation is shown in (D) (n = 3, mean ± SD, *p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Neuropilin-1 by Western Blot

Detection of Neuropilin-1 by Western Blot

Rescue of VEGF-A Signaling in Nrp1cyto and Nrp1fl/fl Primary EC by Full-Length NRP1 and of EC Tubulogenesis following NRP1 siRNA Knockdown with Constitutively Active ERK(A and B) Western blot analysis of Nrp1cyto EC that were transduced with the indicated adenoviral constructs, serum-starved and stimulated with 50 ng/ml VEGF-A165. Blots (A) and quantitation of ERK activation (B) show that Ad-Nrp1, but not Ad-Nrp1cyto or Ad-Nrp1PDZ, restores VEGF-A-induced VEGFR2 and ERK activation in Nrp1cyto EC (mean ± SD, n = 3, *p < 0.05).(C and D) Western blot analysis of Nrp1fl/fl EC treated with adenoviral constructs expressing CRE recombinase to inactivate NRP1 were transduced with the indicated adenoviral constructs, serum-starved and stimulated with 50 ng/ml VEGF-A165. Blots (C) and quantitation (D) show that Ad-Nrp1, but not Ad-Nrp1cyto or Ad-Nrp1PDZ, restores the activation of VEGFR2 and ERK (mean ± SD, n = 3, *p < 0.05).(E and F) HUVEC treated with control or NRP1 siRNA were treated with the indicated adenoviral vectors to compare their capacity to undergo tube formation in 3D collagen matrices after 72 hr. Representative images (E) and quantification (F) of tubulogenesis after 72 hr (mean ± SEM, n = 18, *p < 0.05). Scale bar represents 100 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse/Rat Neuropilin‑1 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of Recombinant Human VEGF165 (Catalog # 293-VE) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566‑NNS) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

Also, 1-5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-NNS) coated at 2 µg/mL (100 µL/well).

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line and Rat cortical stem cells

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.), perfusion fixed frozen sections of rat spinal cord, and immersion fixed paraffin-embedded sections of rat brain (hippocampus and thalamus)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Neuropilin‑1 (Catalog # 5994-N1) and Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1)

Reviewed Applications

Read 12 reviews rated 4.2 using AF566 in the following applications:

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuropilin-1

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Gene Symbol

NRP1

UniProt

Q9QWJ9

Additional Neuropilin-1 Products

Product Documents for Mouse/Rat Neuropilin‑1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse/Rat Neuropilin‑1 Antibody

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

For research use only

Citations for Mouse/Rat Neuropilin‑1 Antibody

Customer Reviews for Mouse/Rat Neuropilin‑1 Antibody (12)

4.2 out of 5
12 Customer Ratings
5 Stars
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Showing  1 - 5 of 12 reviews Showing All
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  • Mouse/Rat Neuropilin-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E12.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/03/2020
    Antibody was stained on E12.5 mouse sections (attached picture) as well as E9.5 mouse sections. Worked well. Note the positive stain in the arteries and the negative signal in the adjacent veins. Dilution used - 1:20
    Mouse/Rat Neuropilin‑1 Antibody AF566
  • Mouse/Rat Neuropilin-1 Antibody
    Name: Alina Filatova
    Application: Immunohistochemistry
    Sample Tested: paraffin-embedded brain
    Species: Mouse
    Verified Customer | Posted 01/23/2019
    The antibody is very sensitive to fixation and epitope retrieval conditions. Many conditions need to be tested to achieve an optimal staining. Testing several dilutions with each sample is recommended.
    Mouse/Rat Neuropilin‑1 Antibody AF566
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22927827
    Species: Other
    Verified Customer | Posted 01/08/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 24034253
    Species: Mouse
    Verified Customer | Posted 01/08/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23406903
    Species: Mouse
    Verified Customer | Posted 01/08/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 24021649
    Species: Mouse
    Verified Customer | Posted 01/08/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23602477
    Species: Mouse
    Verified Customer | Posted 01/08/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Primary mouse hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Hippocampal neurons and astrocytes
    Species: Mouse
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: RN22 cells
    Species: Rat
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse primary cultures
    Species: Mouse
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: a number of human cell lines
    Species: Human
    Verified Customer | Posted 12/18/2014

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