Mouse/Rat Neuropilin-1 Antibody

Catalog # Availability Size / Price Qty
AF566
AF566-SP
Detection  of Recombinant Human, Mouse, and Rat Neuropilin‑1 by Western Blot.
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Product Details
Citations (38)
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Mouse/Rat Neuropilin-1 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
Phe22-Ala810 (Lys811Arg), Ser829-Asp854
Accession # Q9QWJ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
3-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of Recombinant Human VEGF165 (Catalog # 293-VE) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566‑NNS) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

Also, 1-5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-NNS) coated at 2 µg/mL (100 µL/well).

 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Recombinant Human, Mouse, and Rat Neuropilin‑1 by Western Blot. View Larger

Detection of Recombinant Human, Mouse, and Rat Neuropilin‑1 by Western Blot. Western blot shows 25 ng of Recombinant Mouse Neuropilin‑1 (Catalog # 5994-N1), Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin‑1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin‑1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Neuropilin-1 in bEnd.3 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Neuropilin-1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunohistochemistry Neuropilin‑1 in Embryonic Rat Spinal Cord. View Larger

Neuropilin‑1 in Embryonic Rat Spinal Cord. Neuropilin‑1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti‑Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Neuropilin‑1 in Rat Spinal Cord. View Larger

Neuropilin‑1 in Rat Spinal Cord. Neuropilin‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Neuropilin‑1  in Rat Brain. View Larger

Neuropilin‑1 in Rat Brain. Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (hippocampus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Neuropilin‑1  in Rat Brain. View Larger

Neuropilin‑1 in Rat Brain. Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-1

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

Entrez Gene IDs
8829 (Human); 18186 (Mouse); 246331 (Rat)
Alternate Names
BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NRP1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304

Product Datasheets

Citations for Mouse/Rat Neuropilin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Neuropilin 1 (NRP1) hypomorphism combined with defective VEGF-A binding reveals novel roles for NRP1 in developmental and pathological angiogenesis.
    Authors: Fantin, Alessand, Herzog, Birger, Mahmoud, Marwa, Yamaji, Maiko, Plein, Alice, Denti, Laura, Ruhrberg, Christia, Zachary, Ian
    Development, 2104;141(3):556-62.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  2. Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif
    Authors: JM Pinskey, NE Franks, AN McMellen, RJ Giger, BL Allen
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation
    Authors: P Fern ndez-, S Selvam, MB Powner, DA Sim, M Fruttiger
    PLoS ONE, 2017;12(1):e0169865.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Suppression of Retinal Neovascularization by Inhibition of Galectin-1 in a Murine Model of Oxygen-Induced Retinopathy
    Authors: N Yang, W Zhang, T He, Y Xing
    J Ophthalmol, 2017;2017(0):5053035.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  5. Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy
    Authors: N Yang, W Zhang, T He, Y Xing
    Exp. Eye Res, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses
    Authors: K Inokuchi, F Imamura, H Takeuchi, R Kim, H Okuno, H Nishizumi, H Bito, T Kikusui, H Sakano
    Nat Commun, 2017;8(0):15977.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  7. Rewiring the taste system
    Authors: H Lee, LJ Macpherson, CA Parada, CS Zuker, NJP Ryba
    Nature, 2017;548(7667):330-333.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. NOVA2-mediated RNA regulation is required for axonal pathfinding during development
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  9. Neuropilin-1 and the Positions of Glomeruli in the Mouse Olfactory Bulb
    Authors: Peter Mombaerts
    eNeuro, 2016;3(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  10. VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain.
    Authors: Tillo M, Erskine L, Cariboni A, Fantin A, Joyce A, Denti L, Ruhrberg C
    Development, 2015;142(2):314-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  11. Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation.
    Authors: Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C
    J Clin Invest, 2015;125(7):2661-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  12. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
    Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
    Nat Med, 2015;21(5):483-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  13. Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A.
    Authors: Giacobini P, Parkash J, Campagne C, Messina A, Casoni F, Vanacker C, Langlet F, Hobo B, Cagnoni G, Gallet S, Hanchate N, Mazur D, Taniguchi M, Mazzone M, Verhaagen J, Ciofi P, Bouret S, Tamagnone L, Prevot V
    PLoS Biol, 2014;12(3):e1001808.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats.
    Authors: Ezkurdia N, Raurell I, Rodriguez S, Gonzalez A, Esteban R, Genesca J, Martell M
    PLoS ONE, 2014;9(1):e84374.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  15. Fluorochrome-based definition of naturally occurring Foxp3(+) regulatory T cells of intra- and extrathymic origin.
    Authors: Petzold C, Steinbronn N, Gereke M, Strasser R, Sparwasser T, Bruder D, Geffers R, Schallenberg S, Kretschmer K
    Eur J Immunol, 2014;44(12):3632-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  16. Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells.
    Authors: Raimondi C, Fantin A, Lampropoulou A, Denti L, Chikh A, Ruhrberg C
    J Exp Med, 2014;211(6):1167-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  17. Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing.
    Authors: Lee, Mike, Kiefel, Helena, LaJevic, Melissa, Macauley, Matthew, Kawashima, Hiroto, O'Hara, Edward, Pan, Junliang, Paulson, James C, Butcher, Eugene C
    Nat Immunol, 2014;15(10):982-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
    Authors: Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R
    Lab Invest, 2014;94(7):752-65.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  19. Id2 and Id3 maintain the regulatory T cell pool to suppress inflammatory disease.
    Authors: Miyazaki, Masaki, Miyazaki, Kazuko, Chen, Shuwen, Itoi, Manami, Miller, Marina, Lu, Li-Fan, Varki, Nissi, Chang, Aaron N, Broide, David H, Murre, Cornelis
    Nat Immunol, 2014;15(8):767-76.
  20. Astrocyte-encoded positional cues maintain sensorimotor circuit integrity.
    Authors: Molofsky A, Kelley K, Tsai H, Redmond S, Chang S, Madireddy L, Chan J, Baranzini S, Ullian E, Rowitch D
    Nature, 2014;509(7499):189-94.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neut
  21. Heparin binding VEGF isoforms attenuate hyperoxic embryonic lung growth retardation via a FLK1-neuropilin-1-PKC dependent pathway.
    Authors: Esquibies A, Karihaloo A, Quaggin S, Bazzy-Asaad A, Cantley L
    Respir Res, 2014;15(0):32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Func
  22. T cells generated in the absence of a thoracic thymus fail to establish homeostasis.
    Authors: Smolarchuk C, Zhu L, Chan W, Anderson C
    Eur J Immunol, 2014;44(8):2263-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  23. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
    Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
    Arterioscler Thromb Vasc Biol, 2013;33(5):911-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  24. Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis.
    Authors: Delgoffe, Greg M, Woo, Seng-Ryo, Turnis, Meghan E, Gravano, David M, Guy, Cliff, Overacre, Abigail, Bettini, Matthew, Vogel, Peter, Finkelstein, David, Bonnevier, Jody, Workman, Creg J, Vignali, Dario A
    Nature, 2013;501(7466):252-6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: IP
  25. Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth.
    J. Exp. Med., 2012;209(11):2001-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  26. Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model.
    Authors: Chakraborty G, Kumar S, Mishra R
    PLoS ONE, 2012;7(3):e33633.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  27. Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-kappaB activation and tumor sphere formation.
    Authors: Glinka Y, Mohammed N, Subramaniam V, Jothy S
    Biochem. Biophys. Res. Commun., 2012;425(4):775-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  28. Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells.
    J. Exp. Med., 2012;209(10):1723.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  29. IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression.
    Authors: Milpied P, Massot B, Renand A, Diem S, Herbelin A, Leite-de-Moraes M, Rubio MT, Hermine O
    Blood, 2011;118(11):2993-3002.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  30. Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
    Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
    J. Clin. Invest., 2010;120(5):1694-707.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  31. Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis.
    Authors: Robinson SD, Reynolds LE, Kostourou V, Reynolds AR, da Silva RG, Tavora B, Baker M, Marshall JF, Hodivala-Dilke KM
    J. Biol. Chem., 2009;284(49):33966-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  32. BIG-2 mediates olfactory axon convergence to target glomeruli.
    Authors: Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
    Neuron, 2008;57(6):834-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  33. VEGF promotes vascular sympathetic innervation.
    Authors: Marko SB, Damon DH
    Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2646-52.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB
  34. Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
    Authors: Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M
    J. Clin. Invest., 2008;118(6):2062-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  35. Semaphorin and neuropilin co-expression in motoneurons sets axon sensitivity to environmental semaphorin sources during motor axon pathfinding.
    Authors: Moret F, Renaudot C, Bozon M, Castellani V
    Development, 2007;134(24):4491-501.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  36. Modulating Sema3A signal with a L1 mimetic peptide is not sufficient to promote motor recovery and axon regeneration after spinal cord injury.
    Authors: Mire E, Thomasset N, Jakeman LB, Rougon G
    Mol. Cell. Neurosci., 2007;37(2):222-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Gating of Sema3E/PlexinD1 signaling by neuropilin-1 switches axonal repulsion to attraction during brain development.
    Authors: Chauvet S, Cohen S, Yoshida Y, Fekrane L, Livet J, Gayet O, Segu L, Buhot MC, Jessell TM, Henderson CE, Mann F
    Neuron, 2007;56(5):807-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  38. The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis.
    Authors: Fantin A, Vieira J, Plein A, Maden C, Ruhrberg C
    Nat Protoc, 0;8(2):418-29.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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