Mouse/Rat Neuropilin-1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB566A
Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (4)
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Mouse/Rat Neuropilin-1 APC-conjugated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant human Neuropilin‑1 is observed, and less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
Phe22-Ala810 (Lys811Arg), Ser829-Asp854
Accession # Q9QWJ9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Neuropilin-1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Neuropilin-1 antibody in PC-12 Rat Cell Line antibody by Flow Cytometry. View Larger

Detection of Neuropilin‑1 in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Neuropilin-1

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

Entrez Gene IDs
8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names
BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NP1; NRP; NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R

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Product Specific Notices

* This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Mouse/Rat Neuropilin-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Neuropilin-1 Mediated Arterial Differentiation of Murine Pluripotent Stem Cells
    Authors: D Kim, V Lee, T Dorsey, L Niklason, L Gui, G Dai
    Stem Cells Dev., 2018-03-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  2. FOXP3+ Tregs require WASP to restrain Th2-mediated food allergy
    J Clin Invest, 2016-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. An increase in tolerogenic dendritic cell and natural regulatory T cell numbers during experimental autoimmune encephalomyelitis in Rras-/- mice results in attenuated disease.
    Authors: Ray A, Basu S, Miller N, Chan A, Dittel B
    J Immunol, 2014-04-25;192(11):5109-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice.
    Authors: Xie J, Wu T, Xu K, Huang IK, Cleaver O, Huang CL
    Am. J. Pathol., 2009-07-30;175(3):1315-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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