Rat Neuropilin-1 Biotinylated Antibody

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BAF566
Product Details
Citations (8)
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Rat Neuropilin-1 Biotinylated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Neuropilin-1 extracellular domain in Western blots. In this format, less than 1% cross-reactivity with recombinant rat Neuropilin‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
Phe22-Ala810,Arg,Ser829-Asp854
Accession # Q9QWJ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Neuropilin‑1/BDCA4 Fc Chimera (Catalog # 566-N1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The mature 901 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains, two F5/8 type C domains with homology to coagulation factors V and VIII and a MAM (meprin) domain. In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5 - 7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 CUB domains (8). The heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the F5/8 type C domains (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF165 binding (1). Sema3A signaling can be blocked by VEGF165, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

References
  1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. 
  2. Gu, C. et al. (2003) Dev. Cell 5:45. 
  3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
  4. Soker, S. et al. (1998) Cell 92:735.
  5. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. 
  6. Cackowski, F.C. et al. (2004) Genomics 84:82. 
  7. Rossignol, M. et al. (2000) Genomics 70:211.
  8. Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
  9. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
  10. Yaron, A. et al. (2005) Neuron 45:513.
  11. Narazaki, M. and G. Tosato (2006) Blood 107:3892.
  12. Tordjman, R. et al. (2002) Nat. Immunol. 3477.
Entrez Gene IDs
8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names
BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NP1; NRP; NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to >obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Rat Neuropilin-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. STIM1 controls T cell–mediated immune regulation and inflammation in chronic infection
    Authors: Ludovic Desvignes, Carl Weidinger, Patrick Shaw, Martin Vaeth, Theo Ribierre, Menghan Liu et al.
    Journal of Clinical Investigation
  2. Compartmentalized gut lymph node drainage dictates adaptive immune responses
    Authors: D Esterházy, MCC Canesso, L Mesin, PA Muller, TBR de Castro, A Lockhart, M ElJalby, AMC Faria, D Mucida
    Nature, 2019-04-15;0(0):.
    Species: Mouse
    Sample Types:
  3. Reciprocal Expression of IL-35 and IL-10 Defines Two Distinct Effector Treg Subsets that Are Required for Maintenance of Immune Tolerance
    Authors: X Wei, J Zhang, Q Gu, M Huang, W Zhang, J Guo, X Zhou
    Cell Rep, 2017-11-14;21(7):1853-1869.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Activation and Functional Specialization of Regulatory T Cells Lead to the Generation of Foxp3 Instability
    Authors: Z Zhang, W Zhang, J Guo, Q Gu, X Zhu, X Zhou
    J. Immunol, 2017-02-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Tr1 Cells, but Not Foxp3+ Regulatory T Cells, Suppress NLRP3 Inflammasome Activation via an IL-10-Dependent Mechanism.
    Authors: Yao Y, Vent-Schmidt J, McGeough M, Wong M, Hoffman H, Steiner T, Levings M
    J Immunol, 2015-06-08;195(2):488-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Transient Treg-cell depletion in adult mice results in persistent self-reactive CD4(+) T-cell responses.
    Authors: Nystrom S, Bourges D, Garry S, Ross E, van Driel I, Gleeson P
    Eur J Immunol, 2014-11-02;44(12):3621-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A.
    Authors: Romi E, Gokhman I, Wong E, Antonovsky N, Ludwig A, Sagi I, Saftig P, Tessier-Lavigne M, Yaron A
    Nat Commun, 2014-06-05;5(0):4058.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells.
    J. Exp. Med., 2012-09-10;209(10):1723.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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