Mouse/Rat Neuropilin‑1 PerCP‑conjugated Antibody

  
  • Specificity
    Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant human Neuropilin‑1 is observed, and less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
    Phe22-Ala810 (Lys811Arg), Ser829-Asp854
    Accession # Q9QWJ9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse/Rat Neuropilin‑1 PerCP‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566C, filled histogram) or isotype control antibody (Catalog # IC108C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Neuropilin‑1 in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Mouse/Rat Neuropilin‑1 PerCP‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566C, filled histogram) or isotype control antibody (Catalog # IC108C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Neuropilin-1

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

  • Entrez Gene IDs:
    8829 (Human); 18186 (Mouse); 246331 (Rat)
  • Alternate Names:
    BDCA-4; CD304; NRP1; BDCA4; CD304 antigen; DKFZp686A03134; DKFZp781F1414; neuropilin 1; neuropilin-1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG
PerCP-conjugated Control

Ctrl IC108C
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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