Mouse/Rat NrCAM Antibody

Catalog # Availability Size / Price Qty
AF8538
Detection of Mouse and Rat NrCAM by Western Blot.
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Mouse/Rat NrCAM Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat NrCAM in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human NrCAM is observed.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NrCAM
Leu30-Pro630
Accession # Q810U4
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat NrCAM antibody by Western Blot. View Larger

Detection of Mouse and Rat NrCAM by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse/Rat NrCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8538) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NrCAM at approximately 104-145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 °C under sterile conditions after opening.

Background: NrCAM

NgCAM related cell adhesion molecule (NrCAM), also known as Bravo, belongs to the L1 family of cell adhesion molecules, which also include L1, neurofascin and close homolog of L1 (CHL-1) (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like domains in their extracellular domain. They also shared a conserved cytoplasmic region containing an ankyrin-binding site. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (1, 2). NrCam mediates homophilic adhesion as well as heterophilic adhesion with a number of neuronal adhesion molecules including contactin, TAG-1/contactin-2, neurofascin and receptor tyrosine phosphatase beta (RPTP beta ) (3‑5). NrCAM has been implicated in the axogenesis of multiple neuronal populations including sensory axons and commissural axons in the spinal cord of chick embryos. It also plays a role in cerebellar granule cell development, and is required in node of Ranvier formation (5, 6).

References
Hortsch, M. (2000) Mol. Cell. Neurosci. 15:1. Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165. Mauro, V.P. et al. (1992) 119:191. Fustig, M. et al. (1999) Dev. Bio. 209:340. Sakurai, T. et al. (2001) J. Cell Bio. 154:1259. Custer, A.W. et al. (2003) J. Neuroscience 23:10032.