Plexin-B3 (also plexin-6) is a 260-270 kDa member of the plexin-B subfamily, plexin family of proteins. It is found on postnatal neurons and oligodendroglia, and appears to have opposing functions. In a context-dependent manner, plexin-B3 may block cell process extension and induce growth cone collapse by binding to Sema5. Conversely, it may engage in homophilic interactions in trans, promoting growth cone extension and axon elongation. The HGF receptor Met has been reported to complex with plexin-B3 in cis, and this interaction may contribute to its reported opposing effects. Mature mouse plexin-B3 is an 1868 amino acid (aa) type I transmembrane glycoprotein. It contains a 1211 aa extracellular domain (ECD) (aa 25-1892) plus a 636 aa cytoplasmic region. The ECD contains one Sema-domain (aa 25-451), two PSI domains (aa 454-646) and four IPT regions (aa 814-1224). There is one isoform variant that contains an alternative Met start site 10 aa upstream of the standard site. Based on human studies, proteolysis of mouse plexin-B3 likely occurs, generating 160 kDa and 140 kDa ECD fragments. Over aa 25-1235, mouse plexin-B3 shares 95% and 82% aa identity with rat and human plexin-B3, respectively.
Mouse/Rat Plexin B3 Antibody
R&D Systems | Catalog # AF6879
Discontinued Product
AF6879 has been discontinued.
View all Plexin B3 products.
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin B3
His25-Gln1235
Accession # Q9QY40
His25-Gln1235
Accession # Q9QY40
Specificity
Detects mouse and rat Plexin B3 in direct ELISAs and Western blots. In direct ELISAs, approximately 38% cross-reactivity with recombinant human (rh) Plexin B3 is observed, and less than 1% cross-reactivity with rhPlexin B2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse/Rat Plexin B3 Antibody
Detection of Mouse Plexin B3 by Western Blot.
Western blot shows lysates of mouse brain (thalamus/hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6879) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Plexin B3 at approximately 180-190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Plexin B3 in Neuro‑2A Mouse Cell Line by Flow Cytometry.
Neuro-2A mouse neuroblastoma cell line was stained with Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6879, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
Detection of Plexin B3 in C6 Rat Cell Line by Flow Cytometry.
C6 rat glioma cell line was stained with Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6879, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
Plexin B3 in Mouse Brain.
Plexin B3 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6879) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to oligodendrocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse/Rat Plexin B3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Neuro‑2A mouse neuroblastoma cell line and C6 rat glioma cell line
Sample: Neuro‑2A mouse neuroblastoma cell line and C6 rat glioma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain
Sample: Perfusion fixed frozen sections of mouse brain
Western Blot
1 µg/mL
Sample: Mouse brain (thalamus/hypothalamus) tissue
Sample: Mouse brain (thalamus/hypothalamus) tissue
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Plexin B3
Alternate Names
PLEXB3, PLEXR, PLXN6, PLXNB3
Gene Symbol
PLXNB3
UniProt
Additional Plexin B3 Products
Product Documents for Mouse/Rat Plexin B3 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse/Rat Plexin B3 Antibody
For research use only
Citations for Mouse/Rat Plexin B3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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