Mouse/Rat/Primate Phospho-Tyrosine Hydroxylase (S31) Antibody

Catalog # Availability Size / Price Qty
Detection of Phospho-Tyrosine Hydroxylase (S31) by Western Blot
1 Image
Product Details
Citations (2)
Supplemental Products

Mouse/Rat/Primate Phospho-Tyrosine Hydroxylase (S31) Antibody Summary

Species Reactivity
Mouse, Rat, Primate
Mouse and rat ~62 kDa Tyrosine Hydroxylase phosphorylated at S31
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S31 of Tyrosine Hydroxylase
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.


Recommended Concentration
Western Blot
1:1000 dilution
See below
1:1000 dilution
(frozen sections)
1:1000 dilution
(frozen sections)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-Tyrosine Hydroxylase (S31) antibody by Western Blot View Larger

Detection of Phospho-Tyrosine Hydroxylase (S31) by Western Blot Western blot of PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 μM for 60 minutes) showing specific immunolabeling of the approximately 62 kDa Tyrosine Hydroxylase phosphorylated at S31.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Tyrosine Hydroxylase

Rat tyrosine hydroxylase (TH) is a pterin-dependent monooxygenase that catalyzes the hydroxylation of tyrosine to DOPA through the use of a non-heme iron. It is a 55 kDa, 498 amino acid (aa) alpha -helical protein that runs anomalously at 62 kDa in SDS-PAGE. The molecule contains a 164 aa N-terminal regulatory region and a 334 aa C-terminal catalytic domain. Its activity is regulated at the post-transcriptional level by phosphorylation of serine and feedback by catecholamines. Four serines are known to be phosphorylated at aa positions 8, 19, 31, and 40. Different kinases contribute to different phosphorylation patterns. For example, MAPKAPK-2 and CaMKII act on S19 and S40, PKA phosphorylates only at S40, while Cdk5 phosphorylates S31. Variable site phosphorylations have variable effects. S40 phosphorylation blocks catecholamine feedback inhibition. Subsequent phosphorylations at S19 or S31 likely stabilize an otherwise unstable enzyme phosphorylated only at S40, contributing to increased enzyme activity. Phosphorylation at S8 is likely to be physiologically unimportant.

  1. Grima, B. et al. (1985) Proc. Natl. Acad. Sci. USA 82:617.
  2. Frantom, P.A. et al. (2006) Biochemistry 45:2372.
  3. Moy, L.Y. and L-H. Tsai (2004) J. Biol. Chem. 279:54487.
  4. Royo, M. et al. (2005) Arch. Biochem. Biophys. 434:266.
  5. Witkovsky, P. et al. (2000) J. Chem. Neuroanat. 19:105.
Entrez Gene IDs
7054 (Human); 21823 (Mouse); 25085 (Rat)
Alternate Names
DYT14; DYT5b; EC 1.14.16; EC; TH; TYH dystonia 14; TYH; Tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; Tyrosine Hydroxylase

Product Datasheets

You must select a language.


Citations for Mouse/Rat/Primate Phospho-Tyrosine Hydroxylase (S31) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. The protective effect of biochanin A against rotenone-induced neurotoxicity in mice involves enhancing of PI3K/Akt/mTOR signaling and beclin-1 production
    Authors: NA El-Sherbee, N Soliman, AM Youssef, NM Abd El-Fad, TB El-Abaseri, AA Hashish, WK Abdelbasse, G El-Saber B, SA Zaitone
    Ecotoxicol. Environ. Saf., 2020-09-22;205(0):111344.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Cyclin-dependent kinase 5 in the ventral tegmental area regulates depression-related behaviors.
    Authors: Zhong, Peng, Liu, Xiaojie, Zhang, Zhen, Hu, Ying, Liu, Sarah J, Lezama-Ruiz, Martha, Joksimovic, Milan, Liu, Qing-son
    J Neurosci, 2014-04-30;34(18):6352-66.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse/Rat/Primate Phospho-Tyrosine Hydroxylase (S31) Antibody

There are currently no reviews for this product. Be the first to review Mouse/Rat/Primate Phospho-Tyrosine Hydroxylase (S31) Antibody and earn rewards!

Have you used Mouse/Rat/Primate Phospho-Tyrosine Hydroxylase (S31) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review