Key Product Details

Species Reactivity

Mouse, Rat

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 772734
View all SIRP alpha/CD172a Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat SIRP alpha /CD172a
Lys32-Asn373
Accession # P97710

Specificity

Detects rat SIRP alpha /CD172a in direct ELISAs and Western blots. Detects mouse and rat SIRP alpha /CD172a in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Mouse/Rat SIRP alpha /CD172a APC‑conjugated Antibody

Detection of SIRPa/CD172a antibody in PC-12 Rat Cell Line antibody by Flow Cytometry.

Detection of SIRP alpha /CD172a in PC‑12 Rat Cell Line by Flow Cytometry.

PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Mouse/Rat SIRPa/CD172a APC-conjugated Monoclonal Antibody (Catalog # FAB7307A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of SIRPa/CD172a antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of SIRP alpha /CD172a in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB1124P) and either (A) Mouse Anti-Mouse/Rat SIRPa/CD172a APC-conjugated Monoclonal Antibody (Catalog # FAB7307A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse/Rat SIRP alpha /CD172a APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: PC‑12 rat adrenal pheochromocytoma cell line and mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SIRP alpha/CD172a

SIRP alpha (Signal regulatory protein alpha; also known as CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha, respectively.

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Entrez Gene IDs

140885 (Human); 19261 (Mouse); 25528 (Rat); 494566 (Porcine); 609452 (Canine); 101926317 (Cynomolgus Monkey)

Gene Symbol

SIRPA

UniProt

P97710

Additional SIRP alpha/CD172a Products

Product Documents for Mouse/Rat SIRP alpha /CD172a APC‑conjugated Antibody

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Product Specific Notices for Mouse/Rat SIRP alpha /CD172a APC‑conjugated Antibody

For research use only

Citations for Mouse/Rat SIRP alpha /CD172a APC‑conjugated Antibody

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