Mouse/Rat SIRP alpha /CD172a Alexa Fluor® 488-conjugated Antibody
Mouse/Rat SIRP alpha /CD172a Alexa Fluor® 488-conjugated Antibody Summary
Accession # P97710
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of SIRP alpha /CD172a in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Mouse/Rat SIRPa/CD172a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7307G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of SIRP alpha /CD172a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Integrin aM/CD11b APC-conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Mouse Anti-Mouse/Rat SIRPa/CD172a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7307G) or (B) Mouse IgG2BAlexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also known as CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha, respectively.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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