Mouse/Rat SIRP alpha /CD172a Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects rat SIRP alpha /CD172a in direct ELISAs and Western blots. Detects mouse and rat SIRP alpha /CD172a in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 772734
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat SIRP alpha /CD172a
    Lys32-Asn373
    Accession # P97710
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SIRP alpha /CD172a in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Mouse/Rat SIRP alpha /CD172a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7307G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of SIRP alpha /CD172a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Integrin  alpha M/CD11b APC‑conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Mouse Anti-Mouse/Rat SIRP alpha /CD172a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7307G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also known as CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha, respectively.
  • Long Name:
    Signal-regulatory Protein alpha
  • Entrez Gene IDs:
    140885 (Human); 19261 (Mouse); 25528 (Rat); 101926317 (Cynomolgus Monkey)
  • Alternate Names:
    BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alpha; SIRPA; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; SIRPtyrosine-protein phosphatase non-receptor type substrate 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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