Mouse/Rat SP-D Antibody

Catalog # Availability Size / Price Qty
AF6839
AF6839-SP
Detection of Mouse and Rat SP‑D by Western Blot.
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Mouse/Rat SP-D Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat SP-D in Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human SP-D is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse SP-D
Ala20-Phe374
Accession # P50404
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat SP‑D by Western Blot. View Larger

Detection of Mouse and Rat SP‑D by Western Blot. Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse/Rat SP‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6839) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SP‑D at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Rat and Mouse SP‑D by Simple WesternTM. View Larger

Detection of Rat and Mouse SP‑D by Simple WesternTM. Simple Western lane view shows lysates of rat lung tissue and mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for SP‑D at approximately 60 kDa (as indicated) using 20 µg/mL of Sheep Anti-Mouse/Rat SP‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6839) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SP-D

SP‑D (surfactant protein‑D; also PSP‑D) is a 43 kDa member of the collectin family of innate immune modulators (1 ‑ 5). It is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures. SP‑D is found in serum, plasma, broncho‑alveolar lavage (BAL) fluid, and amniotic fluid (1, 2, 6). Lung injuries often increase release of SP‑D to the circulation (3, 6). Mouse SP‑D is synthesized as a 374 amino acid (aa) precursor. Mouse SP‑D cDNA encodes a 19 aa signal sequence and a 355 aa mature region with a 25 aa N‑terminal linking‑region, a 177 aa hydroxyproline and hydroxylysine collagen‑like domain, a 46 aa coiled‑coil segment, and a 106 aa, C‑terminal collectin‑like C‑type lectin domain (CRD) (5). Mature mouse SP‑D shares 72 ‑ 76% aa sequence identity with human, porcine, equine, canine and bovine SP‑D, and 92% with rat SP‑D. SP‑D is usually found as a glycosylated, disulfide‑linked 150 kDa alpha ‑helical coiled‑coil trimer with a “head” of three symmetrical CRDs (2‑4, 7). Each CRD recognizes the hydroxides of one monosaccharide, and trimerization allows for the discrimination of monosaccharide patterns specific to microbial pathogens (4, 7, 8). Typically, SP‑D forms a higher‑order 620 kDa, X‑shaped dodecamer through N‑terminal disulfide bonds, allowing for even finer discrimination of self vs. nonself carbohydrate patterns and facilitating binding to complex antigens (1). SP‑D also binds SIRP alpha and the calreticulin/CD91 complex on macrophages (9, 10). When the ratio of antigen/pathogen to available CRDs is low, antigen can be bound without occupying all available CRDs. The free CRDs will bind to SIRP alpha, generating a signal that downmodulates the inflammatory response. During high CRD ligand binding (low SIRP alpha binding), the dodecamer rearranges to expose N‑termini that bind the calreticulin/CD91 complex, an event that initiates inflammation (1). Also, direct and indirect binding of neutrophil defensins and macrophage CD14 and TLRs to SP‑D can modulate response to viruses and bacterial lipopolysaccharides (1‑3, 11‑15). Thus, SP‑D allows for a graded response to environmental challenge and clearance of small antigenic insults without the need for a damaging inflammatory response (1‑3).

References
  1. Forbes, L.R. and A. Haczku (2010) Clin. Exp. Allergy 40:547.
  2. Kishore, U. et. al. (2006) Mol. Immunol. 43:1293.
  3. Hartl, D. and M. Griese (2006) Eur. J. Clin. Invest. 36:423.
  4. Sim, R.B. et. al. (2006) Novartis Found Symp. 279:170.
  5. Motwani, M. et al. (1995) J. Immunol. 155:5671.
  6. Honda, Y. et al. (1995) Am. J. Respir. Crit. Care Med. 152:1860.
  7. Hakansson, K. et. al. (1999) Structure 7:225.
  8. Crouch, E.C. et. al. (2006) Am. J. Respir. Cell Mol. Biol. 35:84.
  9. Janssen, W.J. et al. (2008) Am. J. Respir. Crit. Care Med. 178:158.
  10. Gardai, S.J. et al. (2003) Cell 115:13.Ohya, M. et. al. (2006) Biochemistry 45:8657.
  11. Ohya, M. et. al. (2006) Biochemistry 45:8657.
  12. Pastva, A.M. et al. (2007) Proc. Am. Thorac. Soc. 4:252.
  13. Sano, H. and Y. Kuroki (2005) Mol. Immunol. 42:279.
  14. Hartshorn, K.L. et al. (2006) J. Immunol. 176:6962.
  15. Yamazoe, M. et al. (2008) J. Biol. Chem. 283:35878.
Long Name
Surfactant Pulmonary Associated Protein D
Entrez Gene IDs
6441 (Human); 20390 (Mouse)
Alternate Names
COLEC7collectin-7; Collectin 7; Collectin-7; Lung surfactant protein D; PSPD; PSP-D; SFTP4; SFTP4pulmonary surfactant-associated protein D; SFTPD; SPD; SP-D; SP-Dpulmonary surfactant apoprotein; surfactant protein D; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4

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