Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all VSIG1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VSIG1
Val23-Glu234
Accession # BAE35812

Specificity

Detects mouse and rat VSIG1 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) VSIG1 is observed and less than 1% cross-reactivity with recombinant mouse (rm) VSIG2, rmVSIG3, rmVSIG4, and rhVSIG3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse/Rat VSIG1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse and Rat VSIG1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG1

VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein 34 and Cell surface antigen 33) is a 55‑70 kDa member of the JAM family of Ig-Superfamily proteins. It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion. Mouse VSIG1 precursor is 407 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD) (aa 23‑234) and a 152 aa cytoplasmic region. The ECD contains one V-type (aa 23‑134) and one C2-type Ig-like domain (aa 145‑229). Over aa 23‑234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively. There is one potential alternate start site at Met108.

Long Name

V-Set and Immunoglobulin Domain Containing 1

Alternate Names

GPA34

Entrez Gene IDs

340547 (Human); 78789 (Mouse); 315920 (Rat); 102123142 (Cynomolgus Monkey)

Gene Symbol

VSIG1

UniProt

BAE35812

Additional VSIG1 Products

Product Documents for Mouse/Rat VSIG1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat VSIG1 Biotinylated Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Inflammatory Mediators

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