Mouse/Rat VSIG1 Antibody

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Detection of Mouse and Rat VSIG1 by Western Blot.
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Product Details
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Mouse/Rat VSIG1 Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat VSIG1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) VSIG1 is observed and less than 1% cross‑reactivity with recombinant mouse (rm) VSIG2, rmVSIG3, rmVSIG4, and rhVSIG3 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse VSIG1
Accession # BAE35812
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat VSIG1 antibody by Western Blot. View Larger

Detection of Mouse and Rat VSIG1 by Western Blot. Western blot shows lysates of rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5324) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for VSIG1 at approximately 55 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG1

VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein 34 and Cell surface antigen 33) is a 55‑70 kDa member of the JAM family of Ig-Superfamily proteins. It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion. Mouse VSIG1 precursor is 407 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD) (aa 23‑234) and a 152 aa cytoplasmic region. The ECD contains one V-type (aa 23‑134) and one C2-type Ig-like domain (aa 145‑229). Over aa 23‑234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively. There is one potential alternate start site at Met108.

Long Name
V-Set and Immunoglobulin Domain Containing 1
Entrez Gene IDs
340547 (Human); 78789 (Mouse); 315920 (Rat); 102123142 (Cynomolgus Monkey)
Alternate Names
Cell surface A33 antigen; dJ889N15.1; Glycoprotein A34; GPA34; MGC44287,1700062D20Rik; V-set and immunoglobulin domain containing 1; V-set and immunoglobulin domain-containing protein 1; VSIG1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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