Mouse RBP4 Quantikine ELISA Kit

R&D Systems | Catalog # MRBP40

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (25 µL)

Sensitivity

0.043 ng/mL

Assay Range

0.625-20 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
View all RBP4/Retinol-Binding Protein 4 ELISA Kits »

Product Summary for Mouse RBP4 Quantikine ELISA Kit

The Quantikine Mouse RBP4 immunoassay is a 4.5 hour solid-phase ELISA designed to measure RBP4 in cell culture supernates, cell lysates, mouse serum, plasma, and urine. It contains NS0-expressed recombinant mouse RBP4 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse RBP4. Results obtained using natural mouse RBP4 showed dose response curves that were parallel to the standard curve obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse RBP4.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse RBP4

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.896 2.23 9.44 0.902 2.22 8.52
Standard Deviation 0.036 0.12 0.51 0.089 0.15 0.64
CV% 4.0 5.4 5.4 9.9 6.8 7.5

Recovery for Mouse RBP4 Quantikine ELISA Kit

The recovery of mouse RBP4 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 98 90-112
Urine (n=4) 91 80-107

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse RBP4 in each matrix were diluted with Calibrator Diluent and assayed.

Mouse RBP4/Retinol-Binding Protein 4 ELISA Linearity

Scientific Data Images for Mouse RBP4 Quantikine ELISA Kit

Mouse RBP4/Retinol-Binding Protein 4 ELISA Standard Curve

Mouse RBP4/Retinol-Binding Protein 4 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: RBP4/Retinol-Binding Protein 4

Retinol-binding protein 4 (RBP4) is a Lipocalin superfamily molecule that transports vitamin A (retinol) and retinaldehyde in the serum. Retinaldehyde is the critical chromophore in the rhodopsin photoreceptor, while both it and retinoic acid regulate a multitude of cellular differentiation and proliferation effects through the intracellular receptors RAR and RXR. RBP4 is secreted primarily by hepatocytes and adipocytes into the blood where the RBP4-retinol complex interacts with transthyretin (TTR). Formation of the complex with TTR increases the serum half-life of RBP4 by preventing RBP4 filtration through the kidney. The C-terminally processed forms of RBP4, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. RBP4 promotes hyperglycemia, and its upregulation in visceral and liver adipocytes leads to elevated serum levels.

Alternate Names

RetinolBinding Protein 4

Entrez Gene IDs

5950 (Human); 19662 (Mouse); 25703 (Rat)

Gene Symbol

RBP4

Additional RBP4/Retinol-Binding Protein 4 Products

Product Documents for Mouse RBP4 Quantikine ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse RBP4 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse RBP4 Quantikine ELISA Kit

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Protocols

View specific protocols for Mouse RBP4 Quantikine ELISA Kit (MRBP40):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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