Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) Reg3A, rmReg3D, recombinant human Reg3D, recombinant rat (rr) Reg3B, or rrReg3G.
Monoclonal Rat IgG2B Clone # 518630
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Reg3B
Glu27-Gly175 Accession # P35230
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Reg3B by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg3B Monoclonal Antibody (Catalog # MAB5110) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Reg3B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reg3B (regenerating islet-derived protein III
beta; also PAP-I and HIP) is a secreted, 17 kDa member of the Reg gene family,
C-type lectin gene superfamily. It is
found in pancreatic acinar cells, intestinal enterocytes and developing spinal
may play a role in intestinal epithelial cell renewal.
It contains one C-type lectin domain. Mature mouse Reg3B
is 91% and 67% aa identical to rat and human Reg3B, respectively.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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